2-Deoxyglucose: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr=2-DG | |abbr=2-DG | ||
|description='''2-Deoxyglucose''', also known as 2-deoxy-D-glucose is a glucose derivative | |description='''2-Deoxyglucose''', also known as 2-deoxy-D-glucose is a glucose derivative that has the 2-hydroxyl group replaced by hydrogen. It competitively inhibits glycolysis by blocking hexokinase and phosphohexoseisomerase. | ||
|info=[[Proton_flux]], [[O2k-pH_ISE-Module]] | |info=[[Proton_flux]], [[O2k-pH_ISE-Module]] | ||
}} | }} | ||
__TOC__ | |||
== Application in [[HRR]] == | |||
:::'''2-DG:ย 2-deoxy-D-glucose'''ย (C<sub>6</sub>H<sub>12</sub>0<sub>5</sub>);ย Sigma D6134, 1 g or 5 g, store at 2-8 ยฐC; M = 164.16 gยทmol<sup>-1</sup> | |||
::::'''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O): | |||
::::# weigh 1 g of 2-deoxy-D-glucose | |||
::::# dissolve with 3.0458 mL H<sub>2</sub>O in a 10 mL-glass beaker | |||
:::ยป'''O2k manual titrations:'''ย [[MiPNet09.12 O2k-Titrations]] | |||
::::* Titration volume: 25 ยตL using a 50 ยตL syringe | |||
::::* Final conc. in 2 mL O2k-chamber: 25 mM | |||
{{MitoPedia concepts}} | {{MitoPedia concepts}} | ||
{{MitoPedia methods}} | {{MitoPedia methods}} | ||
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|mitopedia topic=Inhibitor | |mitopedia topic=Inhibitor | ||
}} | }} | ||
Latest revision as of 11:16, 21 June 2021
Description
2-Deoxyglucose, also known as 2-deoxy-D-glucose is a glucose derivative that has the 2-hydroxyl group replaced by hydrogen. It competitively inhibits glycolysis by blocking hexokinase and phosphohexoseisomerase.
Abbreviation: 2-DG
Reference: Proton_flux, O2k-pH_ISE-Module
Application in HRR
- 2-DG: 2-deoxy-D-glucose (C6H1205); Sigma D6134, 1 g or 5 g, store at 2-8 ยฐC; M = 164.16 gยทmol-1
- Preparation of 2 M stock solution (dissolved in H2O):
- weigh 1 g of 2-deoxy-D-glucose
- dissolve with 3.0458 mL H2O in a 10 mL-glass beaker
- ยปO2k manual titrations: MiPNet09.12 O2k-Titrations
- Titration volume: 25 ยตL using a 50 ยตL syringe
- Final conc. in 2 mL O2k-chamber: 25 mM
MitoPedia topics:
Inhibitor