ADP: Difference between revisions
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Preparation of 500 mM stock solution: 0.491 g/2 ml of final volume. Add 1 ml H2O and 450 ul 5 N KOH. ADP will dissolve. Check pH and adjust to 7, adjust total volume to 2 ml and divide into 0.2 ml portions. Store frozen at -80 °C. | Preparation of 500 mM stock solution: 0.491 g/2 ml of final volume. Add 1 ml H2O and 450 ul 5 N KOH. ADP will dissolve. Check pH and adjust to 7, adjust total volume to 2 ml and divide into 0.2 ml portions. Store frozen at -80 °C. | ||
To keep [Mg2+] constant during respiration measurement mix ADP with MgCl2 (0.6 mol/mol ADP). | |||
Preparation of Mg-ADP: After adjusting pH of ADP to 7, add 121.98 mg of MgCl2 (Scharlau MA0036: MgCl2.6H2O, MW=203.3). White precipitate will occurr, which will be dissolved after 1-2 min of stirring on magnetic stirrer. Adjust final volume to 2 ml, divide into 0.2 ml portions and store at -80 °C. | Preparation of Mg-ADP: After adjusting pH of ADP to 7, add 121.98 mg of MgCl2 (Scharlau MA0036: MgCl2.6H2O, MW=203.3). White precipitate will occurr, which will be dissolved after 1-2 min of stirring on magnetic stirrer. Adjust final volume to 2 ml, divide into 0.2 ml portions and store at -80 °C. | ||
|info=[[MiPNet03.02]], [[MiPNet09.12]], | |info=[[MiPNet03.02]], [[MiPNet09.12]], | ||
Revision as of 10:40, 9 November 2010
- high-resolution terminology - matching measurements at high-resolution
ADP
Description
Adenosine 5’diphosphate (D), C10H15N5O10P2K, potassium salt; substrate of ANT and ATP synthase.
HRR: D: ADP**(Sigma,A 5285: Adenosine 5’diphosphate, C10H15N5O10P2K, potassium salt, contains 1 mol/mol H2O, MW=491.2) Preparation of 500 mM stock solution: 0.491 g/2 ml of final volume. Add 1 ml H2O and 450 ul 5 N KOH. ADP will dissolve. Check pH and adjust to 7, adjust total volume to 2 ml and divide into 0.2 ml portions. Store frozen at -80 °C.
To keep [Mg2+] constant during respiration measurement mix ADP with MgCl2 (0.6 mol/mol ADP). Preparation of Mg-ADP: After adjusting pH of ADP to 7, add 121.98 mg of MgCl2 (Scharlau MA0036: MgCl2.6H2O, MW=203.3). White precipitate will occurr, which will be dissolved after 1-2 min of stirring on magnetic stirrer. Adjust final volume to 2 ml, divide into 0.2 ml portions and store at -80 °C.
Abbreviation: D
Reference: MiPNet03.02, MiPNet09.12,
Labels:
Regulation: ATP; ADP; AMP; PCr"ATP; ADP; AMP; PCr" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
