Difference between revisions of "Adams 2010 Biochim Biophys Acta"
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{{Publication | {{Publication | ||
|title=Adams AE, Kelly OM, Porter RK (2010) Absence of mitochondrial uncoupling protein 1 affects apoptosis in thymocytes, thymocyte/T-cell profile and peripheral T-cell number. Biochim. Biophys. Acta.1797(6-7):807-816. | |title=Adams AE, Kelly OM, Porter RK (2010) Absence of mitochondrial uncoupling protein 1 affects apoptosis in thymocytes, thymocyte/T-cell profile and peripheral T-cell number. Biochim. Biophys. Acta.1797(6-7):807-816. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/20417612 PMID:20417612 | |info=[http://www.ncbi.nlm.nih.gov/pubmed/20417612 PMID:20417612] | ||
|authors=Adams AE, Kelly OM, Porter RK | |authors=Adams AE, Kelly OM, Porter RK | ||
|year=2010 | |year=2010 | ||
|journal=Biochim. Biophys. Acta | |journal=Biochim. Biophys. Acta | ||
|abstract=Our laboratory has previously demonstrated the presence of constitutively expressed mitochondrial uncoupling protein 1 in mouse thymocytes. In our endeavours to understand the role of mitochondrial uncoupling protein 1 in thymocyte function, we compared cell profiles in thymus and spleen of wild-type with those of UCP 1 knock-out mice, which in turn led to comparative investigations of apoptotic potential in thymocytes from these mice. We demonstrate that spleen cell numbers were reduced | |abstract=Our laboratory has previously demonstrated the presence of constitutively expressed mitochondrial uncoupling protein 1 in mouse thymocytes. In our endeavours to understand the role of mitochondrial uncoupling protein 1 in thymocyte function, we compared cell profiles in thymus and spleen of wild-type | ||
with those of UCP 1 knock-out mice, which in turn led to comparative investigations of apoptotic potential in thymocytes from these mice. We demonstrate that spleen cell numbers were reduced βΌ3-fold in UCP 1 knock-out mice compared to wild-type mice. We record a halving of CD8 single positive cell numbers in thymus with a significant incremental increase in CD4/CD8 double positives cell numbers in the thymus of UCP 1 knock-out mice compared to wild-type mice. These data are mirrored by an approximate halving of CD8 single positive cell numbers and a doubling of CD4/CD8 double positive cell numbers in the spleen of UCP 1 knock-out mice compared to wild-type mice. These differences are most probably explained by our observations of decreased apoptotic potential and higher ATP levels in thymocytes of UCP 1 knock-out mice | |||
when compared to wild-type controls. We conclude that constitutively expressed UCP 1 is a factor in determining T-cell population selection in mice. | |||
|keywords=mitochondria, uncoupling protein, thymus, spleen | |keywords=mitochondria, uncoupling protein, thymus, spleen | ||
|mipnetlab=IE_Dublin_Porter_RK | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
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|tissues=Islet Cell; Pancreas; Thymus | |tissues=Islet Cell; Pancreas; Thymus | ||
|preparations=Intact Cell; Cultured; Primary | |preparations=Intact Cell; Cultured; Primary | ||
|additional=mitochondria, uncoupling protein, thymus, spleen | |||
}} | }} |
Revision as of 10:24, 5 September 2011
Adams AE, Kelly OM, Porter RK (2010) Absence of mitochondrial uncoupling protein 1 affects apoptosis in thymocytes, thymocyte/T-cell profile and peripheral T-cell number. Biochim. Biophys. Acta.1797(6-7):807-816. |
Adams AE, Kelly OM, Porter RK (2010) Biochim. Biophys. Acta
Abstract: Our laboratory has previously demonstrated the presence of constitutively expressed mitochondrial uncoupling protein 1 in mouse thymocytes. In our endeavours to understand the role of mitochondrial uncoupling protein 1 in thymocyte function, we compared cell profiles in thymus and spleen of wild-type with those of UCP 1 knock-out mice, which in turn led to comparative investigations of apoptotic potential in thymocytes from these mice. We demonstrate that spleen cell numbers were reduced βΌ3-fold in UCP 1 knock-out mice compared to wild-type mice. We record a halving of CD8 single positive cell numbers in thymus with a significant incremental increase in CD4/CD8 double positives cell numbers in the thymus of UCP 1 knock-out mice compared to wild-type mice. These data are mirrored by an approximate halving of CD8 single positive cell numbers and a doubling of CD4/CD8 double positive cell numbers in the spleen of UCP 1 knock-out mice compared to wild-type mice. These differences are most probably explained by our observations of decreased apoptotic potential and higher ATP levels in thymocytes of UCP 1 knock-out mice when compared to wild-type controls. We conclude that constitutively expressed UCP 1 is a factor in determining T-cell population selection in mice. β’ Keywords: mitochondria, uncoupling protein, thymus, spleen
β’ O2k-Network Lab: IE_Dublin_Porter_RK
Labels:
Organism: Mouse
Tissue;cell: Islet Cell; Pancreas; Thymus"Islet Cell; Pancreas; Thymus" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.
Preparation: Intact Cell; Cultured; Primary"Intact Cell; Cultured; Primary" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
HRR: Oxygraph-2k
mitochondria, uncoupling protein, thymus, spleen