Difference between revisions of "Antimycin A"
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{{MitoPedia | {{MitoPedia | ||
|abbr=Ama | |abbr=Ama | ||
|description='''Antimycin A''' is an inhibitor of [[Complex III]] (CIII). It binds to the Qi site of CIII and inhibits the transfer of electrons from heme ''b''<sub>H</sub> to oxidized Q (Qi site inhibitor). | |description='''Antimycin A''' is an inhibitor of [[Complex III]] (CIII). It binds to the Qi site of CIII and inhibits the transfer of electrons from heme ''b''<sub>H</sub> to oxidized Q (Qi site inhibitor). Antimycin A also inhibits acyl-CoA oxidase. | ||
|info=[[MiPNet09.12]] | |info=[[MiPNet09.12]] | ||
|type=Inhibitor ETS | |type=Inhibitor ETS | ||
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== Application in [[HRFR]] == | == Application in [[HRFR]] == | ||
'''Ama: Antimycin A''' (from ''Streptomyces sp.'') Sigma A 8674, 25 mg, store at -20 °C: FW = 540 | ::: '''Ama: Antimycin A''' (from ''Streptomyces sp.'') Sigma A 8674, 25 mg, store at -20 °C: FW = 540 | ||
<span style="color:#8B008B"> '''Caution:''' '''Very toxic.''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span> | :::: <span style="color:#8B008B"> '''Caution:''' '''Very toxic.''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span> | ||
'''Preparation of 5 mM stock solution''' (dissolved in 99.9% ethanol): | ::: '''Preparation of 5 mM stock solution''' (dissolved in 99.9% ethanol): | ||
:# Weigh 5.4 mg of antimycin A into a small glass vial. Caution: Ama is hygroscopic. | ::::# Weigh 5.4 mg of antimycin A into a small glass vial. Caution: Ama is hygroscopic. | ||
:# Add 2 ml ethanol. | ::::# Add 2 ml ethanol. | ||
:# Divide into 0.2 ml portions in glass vials. | ::::# Divide into 0.2 ml portions in glass vials. | ||
:# Store at -20 °C. | ::::# Store at -20 °C. | ||
'''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | ::: '''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | ||
:* Titration volume: 1 µl using a 10 µl syringe (2 ml O2k-chamber). | ::::* Titration volume: 1 µl using a 10 µl syringe (2 ml O2k-chamber). | ||
:* Final concentration: 2.5 µM. | ::::* Final concentration: 2.5 µM. | ||
| Line 36: | Line 36: | ||
== Antimycin A and ROS production == | == Antimycin A and ROS production == | ||
* Is hydrogen peroxide production measured after inhibition of the Electron transfer-pathway by rotenone and antimycin A of diagnostic significance? - Please, share your evidence (Email to [email protected]). | * Is hydrogen peroxide production measured after inhibition of the Electron transfer-pathway by rotenone and antimycin A of diagnostic significance? - Please, share your evidence (Email to [email protected]). | ||
== Inhibition of acyl-CoA oxidase == | |||
::::* Vamecq J, Schepers L, Parmentier G, Mannaerts GP (1987) Inhibition of peroxisomal fatty acyl-CoA oxidase by antimycin A. Biochem J 248:603-7. - [[Vamecq 1987 Biochem J |»Bioblast link«]] | |||
Revision as of 17:58, 25 February 2018
- high-resolution terminology - matching measurements at high-resolution
Antimycin A
Description
Antimycin A is an inhibitor of Complex III (CIII). It binds to the Qi site of CIII and inhibits the transfer of electrons from heme bH to oxidized Q (Qi site inhibitor). Antimycin A also inhibits acyl-CoA oxidase.
Abbreviation: Ama
Reference: MiPNet09.12
MitoPedia topics: Inhibitor
Application in HRFR
- Ama: Antimycin A (from Streptomyces sp.) Sigma A 8674, 25 mg, store at -20 °C: FW = 540
- Caution: Very toxic. Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
- Preparation of 5 mM stock solution (dissolved in 99.9% ethanol):
- Weigh 5.4 mg of antimycin A into a small glass vial. Caution: Ama is hygroscopic.
- Add 2 ml ethanol.
- Divide into 0.2 ml portions in glass vials.
- Store at -20 °C.
- O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 1 µl using a 10 µl syringe (2 ml O2k-chamber).
- Final concentration: 2.5 µM.
Compare
- Myxothiazol - this CIII inhibitor induces less ROS production but is more expensive than Ama.
Antimycin A and ROS production
- Is hydrogen peroxide production measured after inhibition of the Electron transfer-pathway by rotenone and antimycin A of diagnostic significance? - Please, share your evidence (Email to [email protected]).
Inhibition of acyl-CoA oxidase
- Vamecq J, Schepers L, Parmentier G, Mannaerts GP (1987) Inhibition of peroxisomal fatty acyl-CoA oxidase by antimycin A. Biochem J 248:603-7. - »Bioblast link«
