Difference between revisions of "Antimycin A"
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{{MitoPedia | {{MitoPedia | ||
|abbr=Ama | |abbr=Ama | ||
|description='''Antimycin A''' is an inhibitor of [[Complex III]]. It binds to the Qi site of Complex III and inhibits the transfer of electrons from heme bH to oxidized Q (Qi site inhibitor) | |description='''Antimycin A''' is an inhibitor of [[Complex III]]. It binds to the Qi site of Complex III and inhibits the transfer of electrons from heme bH to oxidized Q (Qi site inhibitor). | ||
|info=[[MiPNet09.12]], | |info=[[MiPNet09.12]], | ||
|type=Inhibitor ETS | |type=Inhibitor ETS | ||
}} | |||
{{MitoPedia methods|type=Inhibitor ETS | |||
}} | }} | ||
{{MitoPedia topics | {{MitoPedia topics | ||
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|type=Inhibitor ETS | |type=Inhibitor ETS | ||
}} | }} | ||
== | == Applications in [[HRR]] == | ||
'''Ama: Antimycin A''' (Antimycin A from ''Streptomyces'' | '''Ama: Antimycin A''' (Antimycin A from ''Streptomyces sp.'') Sigma A 8674, 25 g, store at -20 °C: FW = 540 | ||
<span style="color:#8B008B"> '''Caution:''' '''Very toxic!!! ''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span> | <span style="color:#8B008B"> '''Caution:''' '''Very toxic!!! ''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span> | ||
'''Preparation of 5 mM stock solution''' (dissolved in 99.9% ethanol): | |||
::1) Weigh 5.4 mg of Antimycin A into a small glass vial (Ama is hygroscopic). | |||
::2) Add 2 ml ethanol. | |||
::1) | ::3) Divide into 0.2 ml portions in glass vials. | ||
::2) | ::4) Store at -20 °C. | ||
::3) | |||
::4) | |||
'''Oxygraph-2k Manual Titrations:''' [[MiPNet09.12]] | '''Oxygraph-2k Manual Titrations:''' [[MiPNet09.12]] | ||
::* Titration volume: 1 µl using a 10 µl syringe | ::* Titration volume: 1 µl using a 10 µl syringe. | ||
::* Final conc. in 2 ml O2k- | ::* Final conc. in 2 ml O2k-Chamber: 2.5 µM. |
Revision as of 18:07, 24 January 2014
Description
Antimycin A is an inhibitor of Complex III. It binds to the Qi site of Complex III and inhibits the transfer of electrons from heme bH to oxidized Q (Qi site inhibitor).
Abbreviation: Ama
Reference: MiPNet09.12,
MitoPedia topics: Inhibitor
Applications in HRR
Ama: Antimycin A (Antimycin A from Streptomyces sp.) Sigma A 8674, 25 g, store at -20 °C: FW = 540
Caution: Very toxic!!! Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
Preparation of 5 mM stock solution (dissolved in 99.9% ethanol):
- 1) Weigh 5.4 mg of Antimycin A into a small glass vial (Ama is hygroscopic).
- 2) Add 2 ml ethanol.
- 3) Divide into 0.2 ml portions in glass vials.
- 4) Store at -20 °C.
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 1 µl using a 10 µl syringe.
- Final conc. in 2 ml O2k-Chamber: 2.5 µM.