Difference between revisions of "Antimycin A"
From Bioblast
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{{MitoPedia | {{MitoPedia | ||
|abbr=Ama | |abbr=Ama | ||
|description='''Antimycin A''' is an inhibitor of [[Complex III]]. It binds to the Qi site of Complex III and inhibits the transfer of electrons from heme | |description='''Antimycin A''' is an inhibitor of [[Complex III]]. It binds to the Qi site of Complex III and inhibits the transfer of electrons from heme ''b''<sub>H</sub> to oxidized Q (Qi site inhibitor). | ||
|info=[[MiPNet09.12]] | |info=[[MiPNet09.12]] | ||
|type=Inhibitor ETS | |type=Inhibitor ETS | ||
}} | }} | ||
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'''Preparation of 5 mM stock solution''' (dissolved in 99.9% ethanol): | '''Preparation of 5 mM stock solution''' (dissolved in 99.9% ethanol): | ||
::1) Weigh 5.4 mg of | ::1) Weigh 5.4 mg of antimycin A into a small glass vial (Ama is hygroscopic). | ||
::2) Add 2 ml ethanol. | ::2) Add 2 ml ethanol. | ||
::3) Divide into 0.2 ml portions in glass vials. | ::3) Divide into 0.2 ml portions in glass vials. | ||
Revision as of 23:33, 29 June 2015
- high-resolution terminology - matching measurements at high-resolution
Antimycin A
Description
Antimycin A is an inhibitor of Complex III. It binds to the Qi site of Complex III and inhibits the transfer of electrons from heme bH to oxidized Q (Qi site inhibitor).
Abbreviation: Ama
Reference: MiPNet09.12
MitoPedia topics: Inhibitor
Applications in HRR
Ama: Antimycin A (from Streptomyces sp.) Sigma A 8674, 25 mg, store at -20 °C: FW = 540
Caution: Very toxic!!! Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
Preparation of 5 mM stock solution (dissolved in 99.9% ethanol):
- 1) Weigh 5.4 mg of antimycin A into a small glass vial (Ama is hygroscopic).
- 2) Add 2 ml ethanol.
- 3) Divide into 0.2 ml portions in glass vials.
- 4) Store at -20 °C.
O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 1 µl using a 10 µl syringe (2 ml O2k-chamber).
- Final concentration: 2.5 µM.
