Antimycin A: Difference between revisions

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Antimycin A

Description

Antimycin A is an inhibitor of Complex III. It binds to the Qi site of Complex III and inhibits the transfer of electrons from heme bH to oxidized Q (Qi site inhibitor).

Abbreviation: Ama

Reference: MiPNet09.12,

MitoPedia topics: Inhibitor

Applications in HRR

Ama: Antimycin A (Antimycin A from Streptomyces sp.) Sigma A 8674, 25 g, store at -20 °C: FW = 540

Caution: Very toxic!!! Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.

Preparation of 5 mM stock solution (dissolved in 99.9% ethanol):

1) Weigh 5.4 mg of Antimycin A into a small glass vial (Ama is hygroscopic).

2) Add 2 ml ethanol.

3) Divide into 0.2 ml portions in glass vials.

4) Store at -20 °C.

Oxygraph-2k Manual Titrations: MiPNet09.12

Titration volume: 1 µl using a 10 µl syringe.
Final conc. in 2 ml O2k-Chamber: 2.5 µM.

@@ Line 1: / Line 1: @@
 {{MitoPedia
 |abbr=Ama
-|description='''Antimycin A''' is an inhibitor of [[Complex III]]. It binds to the Qi site of Complex III and inhibits the transfer of electrons from heme bH to oxidized Q (Qi site inhibitor)[http://en.wikipedia.org/wiki/Coenzyme_Q_-_cytochrome_c_reductase#Inhibitors_of_complex_III].
+|description='''Antimycin A''' is an inhibitor of [[Complex III]]. It binds to the Qi site of Complex III and inhibits the transfer of electrons from heme bH to oxidized Q (Qi site inhibitor).
 |info=[[MiPNet09.12]],
 |type=Inhibitor ETS
+}}
+{{MitoPedia methods|type=Inhibitor ETS
 }}
 {{MitoPedia topics
@@ Line 9: / Line 11: @@
 |type=Inhibitor ETS
 }}
-== Application in [[HRR]] ==
+== Applications in [[HRR]] ==
-'''Ama: Antimycin A''' (Antimycin A from ''Streptomyces'' sp.) Sigma A 8674, 25 g, store at -20 °C: FW = 540
+'''Ama: Antimycin A''' (Antimycin A from ''Streptomyces sp.'') Sigma A 8674, 25 g, store at -20 °C: FW = 540
 <span style="color:#8B008B"> '''Caution:''' '''Very toxic!!! ''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span>
+'''Preparation of 5 mM stock solution''' (dissolved in 99.9% ethanol):
-'''Preparation of 5 mM stock solution''' (dissolved in 99.9% Ethanol):
+::1) Weigh 5.4 mg of Antimycin A into a small glass vial (Ama is hygroscopic).
+::2) Add 2 ml ethanol.
-::1) weigh 5.4 mg of Antimycin A into a small glass vial    !Difficult to weigh - appears to be hygroscopic!
+::3) Divide into 0.2 ml portions in glass vials.
-::2) add 2 ml Ethanol
+::4) Store at -20 °C.
-::3) divide into 0.2 ml portions (use glass vials)
-::4) store at -20 °C
 '''Oxygraph-2k Manual Titrations:'''  [[MiPNet09.12]]
-::* Titration volume: 1 µl using a 10 µl syringe
+::* Titration volume: 1 µl using a 10 µl syringe.
-::* Final conc. in 2 ml O2k-chamber: 2.5 µM
+::* Final conc. in 2 ml O2k-Chamber: 2.5 µM.