Ap5A: Difference between revisions

Revision as of 09:15, 17 May 2021

high-resolution terminology - matching measurements at high-resolution

Ap5A

Description

P1,P5-Di(adenosine-5')pentaphosphate (Ap5A) is an inhibitor of adenylate kinase (ADK), the enzyme which rephosphorylates AMP to ADP, consuming ATP (ATP + AMP ↔ 2 ADP).

Abbreviation: Ap5A

Application in HRR

Ap5A: P1,P5-Di(adenosine-5′) pentaphosphate pentasodium salt Sigma D4022, store at -20 °C; molecular weight: 1026.28

Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.

Preparation of 100 mM stock solution (dissolved in H₂O)

Dissolve content of Sigma 2 mg glass vial in 19.49 µL H₂O
Store at -20 °C.

» O2k manual titrations MiPNet09.12 O2k-Titrations

Titration volume (2-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
Final concentration: 50 µM.

References

MitoPedia topics: Inhibitor

@@ Line 10: / Line 10: @@
 :::: '''Ap5A: P1,P5-Di(adenosine-5′) pentaphosphate pentasodium salt''' Sigma D4022, store at -20 °C; molecular weight: 1026.28
 ::::  <span style="color:#8B008B"> '''Caution:''' Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.</span>
-:::: '''Preparation of 100 mM stock solution''' (dissolved in H2O)
-:::::# For a 2 mg glass vial: dissolve in 19.49 µL H2O::::# Store at -20 °C.
+:::: '''Preparation of 100 mM stock solution''' (dissolved in H<sub>2</sub>O)
+::::# Dissolve content of Sigma 2 mg glass vial in 19.49 µL H<sub>2</sub>O
+::::# Store at -20 °C.
 :::» '''O2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]
-::::* Titration volume: 1 µL using a 10 µL syringe (2 mL O2k-Chamber).
+::::* Titration volume ('''2-mL O2k-chamber'''): 1 µL using a 10 µL Hamilton syringe.
 ::::* Final concentration: 50 µM.
 == References ==