Difference between revisions of "Atractyloside"

Latest revision as of 12:47, 12 February 2021

high-resolution terminology - matching measurements at high-resolution

Atractyloside

Description

Atractyloside is an inhibitor of the adenine nucleotide translocator (ANT). It is an extremely toxic glycoside that inhibits oxidative phosphorylation by blocking the transfer of adenosine nucleotides through the mitochondrial membrane.

Abbreviation: Atr

Reference: MiPNet03.02; MiPNet09.12

Application in HRR

Atr: Atractyloside (Atractyloside dipotassium salt, C₃₀H₄₄O₁₆S₂K₂); Sigma A 6882, 250 mg, store at R.T.; M = 802.99 g·mol^-1 containing 2.5 mol H₂O/mol.

Caution: very toxic.

Preparation of 50 mM stock solution (dissolved in H₂0):

Weigh 40.2 mg atractyloside.
Dissolve in 1 ml H₂O (it dissolves better in warm water).
Divide into 0.2 ml aliquots.
Store at -20 °C.

» O2k manual titrations MiPNet09.12 O2k-Titrations

Titration volume: 30 µl using a 50 µl syringe (2 ml O2k-chamber).
Final concentration: 0.75 mM.
Comment: With Atr, a very high concentration is required for full inhibition. Carboxyatractyloside (Cat) is considered to be much more specific than Atr, and the effective concentration required is much much lower.

MitoPedia topics: Inhibitor

@@ Line 1: / Line 1: @@
 {{MitoPedia
 |abbr=Atr
-|description=Inhibitor of [[adenine nucleotide translocator|Adenine nucleotide translocator (ANT)]].
+|description='''Atractyloside''' is an inhibitor of the [[Adenine nucleotide translocator|adenine nucleotide translocator (ANT)]]. It is an extremely toxic glycoside that inhibits oxidative phosphorylation by blocking the transfer of adenosine nucleotides through the mitochondrial membrane.
 |info=[[MiPNet03.02]]; [[MiPNet09.12]]
 |type=Inhibitor PS
@@ Line 7: / Line 7: @@
 {{MitoPedia methods|type=Inhibitor PS
 }}
+__TOC__
+== Application in [[HRR]] ==
+::: '''Atr: Atractyloside'''  (Atractyloside dipotassium salt,  C<sub>30</sub>H<sub>44</sub>O<sub>16</sub>S<sub>2</sub>K<sub>2</sub>); Sigma A 6882,  250 mg, store at R.T.; M = 802.99 g·mol<sup>-1</sup> containing 2.5 mol H<sub>2</sub>O/mol.
+:::: <span style="color:#8B008B"> '''Caution:''' very toxic.</span>
+:::: '''Preparation of 50 mM stock solution''' (dissolved in H<sub>2</sub>0):
+::::# Weigh 40.2 mg atractyloside.
+::::# Dissolve in 1 ml H<sub>2</sub>O (it dissolves better in warm water).
+::::# Divide into 0.2 ml aliquots.
+::::# Store at -20 °C.
+:::» '''O2k manual titrations'''  [[MiPNet09.12 O2k-Titrations]]
+::::* Titration volume: 30 µl using a 50 µl syringe (2 ml O2k-chamber).
+::::* Final concentration: 0.75 mM.
+::::* '''Comment:''' With Atr, a very high concentration is required for full  inhibition. [[carboxyatractyloside|Carboxyatractyloside]] (Cat) is considered to be much more specific than Atr, and the effective concentration required is much much lower.
 {{MitoPedia topics
 |mitopedia topic=Inhibitor
 |type=Inhibitor PS
 }}
-With Atr, a very high concentration is required for full inhibition. [[carboxyatractyloside|Carboxyatractyloside]] (Cat) is considered to be much more specific than Atr, and the effective concentration required is much much lower.