Atractyloside: Difference between revisions
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
'''Atr: Atractyloside''' (Atractyloside dipotassium salt, C<sub>30</sub>H<sub>44</sub>O<sub>16</sub>S<sub>2</sub>K<sub>2</sub>); Sigma A 6882, 250 mg, store at R.T.; FW = 802.99 containing 2.5 mol H<sub>2</sub>O/mol | '''Atr: Atractyloside''' (Atractyloside dipotassium salt, C<sub>30</sub>H<sub>44</sub>O<sub>16</sub>S<sub>2</sub>K<sub>2</sub>); Sigma A 6882, 250 mg, store at R.T.; FW = 802.99 containing 2.5 mol H<sub>2</sub>O/mol. | ||
<span style="color:#8B008B"> '''Caution:''' | <span style="color:#8B008B"> '''Caution:''' very toxic.</span> | ||
'''Preparation of 50 mM stock solution''' (dissolved in H<sub>2</sub>0): | '''Preparation of 50 mM stock solution''' (dissolved in H<sub>2</sub>0): | ||
::1) | ::1) Weigh 40.2 mg atractyloside. | ||
::2) | ::2) Dissolve in 1 ml H<sub>2</sub>O (it dissolves better in warm water). | ||
::3) | ::3) Divide into 0.2 ml aliquots. | ||
::4) | ::4) Store at -20 °C. | ||
'''Oxygraph-2k | '''Oxygraph-2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | ||
::* Titration volume: 30 µl using a 50 µl syringe | ::* Titration volume: 30 µl using a 50 µl syringe (2 ml O2k-chamber). | ||
::* Final | ::* Final concentration: 0.75 mM. | ||
'''Comment:''' With Atr, a very high concentration is required for full inhibition. [[carboxyatractyloside|Carboxyatractyloside]] (Cat) is | '''Comment:''' With Atr, a very high concentration is required for full inhibition. [[carboxyatractyloside|Carboxyatractyloside]] (Cat) is considered to be much more specific than Atr, and the effective concentration required is much much lower. | ||
Revision as of 15:51, 2 May 2014
- high-resolution terminology - matching measurements at high-resolution
Atractyloside
Description
Atractyloside is an inhibitor of the Adenine nucleotide translocator (ANT). It is an extremely toxic glycoside that inhibits oxidative phosphorylation by blocking the transfer of adenosine nucleotides through the mitochondrial membrane.
Abbreviation: Atr
Reference: MiPNet03.02; MiPNet09.12
MitoPedia topics: Inhibitor
Application in HRR
Atr: Atractyloside (Atractyloside dipotassium salt, C30H44O16S2K2); Sigma A 6882, 250 mg, store at R.T.; FW = 802.99 containing 2.5 mol H2O/mol.
Caution: very toxic.
Preparation of 50 mM stock solution (dissolved in H20):
- 1) Weigh 40.2 mg atractyloside.
- 2) Dissolve in 1 ml H2O (it dissolves better in warm water).
- 3) Divide into 0.2 ml aliquots.
- 4) Store at -20 °C.
Oxygraph-2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 30 µl using a 50 µl syringe (2 ml O2k-chamber).
- Final concentration: 0.75 mM.
Comment: With Atr, a very high concentration is required for full inhibition. Carboxyatractyloside (Cat) is considered to be much more specific than Atr, and the effective concentration required is much much lower.
