Author IOC61: Difference between revisions

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Staples, J., Chung, D., Armstrong, C. (2010) Fast in, slow out: Kinetics of mitochondrial metabolic suppression during hibernation entrance and arousal. Mitochondr. Physiol. Network 15.10

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Staples, James F., Chung, Dillon, Armstrong, Christopher (2010) Mitochondr. Physiol. Network

Abstract: During entrance into a torpor bout the whole-animal metabolic rate of mammalian hibernators can decrease by up to 100-fold within a matter of hours. This metabolic suppression corresponds with a rapid decrease in succinate-fuelled state 3 respiration rates of liver mitochondria, measured at 37oC. When isolated from 13-lined ground squirrel (Ictodomys tridecemlineatus) in early entrance (Tb=30oC), respiration decreased by 61% compared with interbout euthermia. Respiration did not decline further in late entrance (Tb=15oC) or steady-state torpor (Tb=5oC). In contrast, during early arousal (Tb=15oC), despite rapid increases in metabolic rate, state 3 respiration did not increase significantly from torpor levels. By the time Tb reached 30oC in late arousal, however, respiration was 3-fold higher than torpor and continued to increase gradually, so that in interbout euthermia it was 6-fold higher than torpor and not significantly different from the summer active state. Preincubation with 2mM isocitrate (removing oxaloacetate inhibition of succinate dehydrogenase) increased respiration only in torpor and early arousal. The “fast” initiation of mitochondrial metabolic suppression during entrance and its “slow” reversal during arousal suggests a mechanism that requires high fairly high body temperatures.

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Organism: Other Mammal"Other Mammal" is not in the list (Human, Pig, Mouse, Rat, Guinea pig, Bovines, Horse, Dog, Rabbit, Cat, ...) of allowed values for the "Mammal and model" property. Tissue;cell: Hepatocyte; Liver"Hepatocyte; Liver" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property. Preparation: Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property. Enzyme: Complex II; Succinate Dehydrogenase"Complex II; Succinate Dehydrogenase" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property.

Event: IOC60"IOC60" is not in the list (A1, A2, A3, A4, B1, B2, B3, B4, C1, C2, ...) of allowed values for the "Event" property.

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 {{Publication
-|title=Author A, Author B (2010) Title of abstract. Mitochondr. Physiol. Network 15.10
+|title=Staples, J., Chung, D., Armstrong, C. (2010) Fast in, slow out: Kinetics of mitochondrial metabolic suppression during hibernation entrance and arousal. Mitochondr. Physiol. Network 15.10
-|authors=Author A, Author B
+|info=http://www.uwo.ca/biology/Faculty/staples/index.htm
+|authors=Staples, James F., Chung, Dillon, Armstrong, Christopher
 |year=2010
 |journal=Mitochondr. Physiol. Network
-|abstract='''Abstract''': Add a short abstract here, including title, authors, affiliations, text (up to 250 words), and 2-6 references. You may edit your abstract any time. Information will be provided on a deadline for editing/submitting final abstracts (including a pdf file in final format).
+|abstract=During entrance into a torpor bout the whole-animal metabolic rate of mammalian hibernators can decrease by up to 100-fold within a matter of hours. This metabolic suppression corresponds with a rapid decrease in succinate-fuelled state 3 respiration rates of liver mitochondria, measured at 37oC. When isolated from 13-lined ground squirrel (Ictodomys tridecemlineatus) in early entrance (Tb=30oC), respiration decreased by 61% compared with interbout euthermia. Respiration did not decline further in late entrance (Tb=15oC) or steady-state torpor (Tb=5oC). In contrast, during early arousal (Tb=15oC), despite rapid increases in metabolic rate, state 3 respiration did not increase significantly from torpor levels. By the time Tb reached 30oC in late arousal, however, respiration was 3-fold higher than torpor and continued to increase gradually, so that in interbout euthermia it was 6-fold higher than torpor and not significantly different from the summer active state. Preincubation with 2mM isocitrate (removing oxaloacetate inhibition of succinate dehydrogenase) increased respiration only in torpor and early arousal. The “fast” initiation of mitochondrial metabolic suppression during entrance and its “slow” reversal during arousal suggests a mechanism that requires high fairly high body temperatures.
-'''Title''': Not capitalized.
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-'''Main text''': Structured into paragraphs without headers. The standard structure of abstracts should be followed as appropriate (Introduction / Methods / Results / Conclusions / References).
-'''Figure''': You may submit one or two figures (jpg format), without caption if full explanation is given in the abstract.
-'''References''' in the text are given by numbers in brackets.  Full references should be numbered and include all authors (family name and initials without punctuation), followed by the year of publication in parentheses, full title, journal name abbreviated with punctuation (italic), volume number followed by a colon, and first and last pages. See abstracts on the MiP website for style – MiP2005/Organisation/Abstracts.
-Tick on appropriate boxes blow in the list of 'Labels', and add additional keywords not covered in these labels.
-An extension is possible in the free text (not more than 2 pages). Further comments may be added in the discussion.
-|info=Link to a selected website
 }}
 {{Labeling
-|discipline=Mitochondrial Physiology
+|discipline=Mitochondrial Physiology, Environmental Physiology; Toxicology
+|organism=Other Mammal
+|tissues=Hepatocyte; Liver
+|preparations=Isolated Mitochondria
+|enzymes=Complex II; Succinate Dehydrogenase
+|kinetics=Temperature
 |articletype=Workshop, MiPNet-online Publication
 |event=IOC60