Azzone 1961 J Biol Chem: Difference between revisions
Harrison DK (talk | contribs) (Created page with "{{Publication |title=Azzone GF, Ernster L (1961) Demonstration of a requirement of high energy phosphate for the aerobic oxidation of succinate in liver mitochondria. J Biol Chem...") |
Harrison DK (talk | contribs) No edit summary |
||
Line 1: | Line 1: | ||
{{Publication | {{Publication | ||
|title=Azzone GF, Ernster L (1961) Demonstration of a requirement of high energy phosphate for the aerobic oxidation of succinate in liver mitochondria. J Biol Chem 236:1518-1525. | |title=Azzone GF, Ernster L (1961) Demonstration of a requirement of high energy phosphate for the aerobic oxidation of succinate in liver mitochondria. J Biol Chem 236:1518-1525. | ||
|info=[http://www.jbc.org/content/236/5/1518.full.pdf+html PMID: 13685481 Open Access] | |info=[http://www.jbc.org/content/236/5/1518.full.pdf+html PMID: 13685481 Open Access] | ||
|authors=Azzone GF, Ernster L | |authors=Azzone GF, Ernster L | ||
|year=1961 | |year=1961 | ||
|journal=J Biol Chem | |journal=J Biol Chem | ||
|abstract=Preincubation of rat liver mitochondria in a sucrose-KCl medium in the presence of 2 to 3 mM arsenate and 0.06 mM Dicumarol or 0.1 mM 2,4dinitrophenol for 3 to 4 minutes results in a marked depression of the succinoxidase capacity. No depression is found when the preincubation is made in the presence of Amytal, cysteine sulfinate, or inorganic phosphate. Addition of adenosine triphosphate after the preincubation stimulates succinate oxidation several-fold. The effect of adenosine triphosphate is not duplicated by cysteine sulfinate, inorganic phosphate, ethylenediaminetetraacetate, adenosine 5’-phosphate, cytidine triphosphate, uridine triphosphate, inosine triphosphate, or guanidine triphosphate. Similar results are obtained with mitochondria pretreated with dinitrophenol and adenosine 5’-phosphate. | |||
The data are consistent with the conclusion that conditions leading to a depletion of the endogenous content of mitochondrial high energy phosphate result in a reversible depression of the succinoxidase capacity. | |||
The concept is developed that the aerobic oxidation of succinate in intact liver mitochondria requires an activation by high energy phosphate. Some implications of this concept regarding the enzymic organization of mitochondrial electron transport and oxidative phosphorylation are discussed. | |||
|keywords=succinate oxidation, high energy phosphate, ATP | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
|organism=Rat | |||
|tissues=Hepatocyte; Liver | |||
|preparations=Isolated Mitochondria | |||
|enzymes=Complex II; Succinate Dehydrogenase | |||
|topics=Respiration; OXPHOS; ETS Capacity, Substrate; Glucose; TCA Cycle, ATP; ADP; AMP; PCr | |||
|additional=Made history | |additional=Made history | ||
}} | }} |
Revision as of 21:17, 12 June 2012
Azzone GF, Ernster L (1961) Demonstration of a requirement of high energy phosphate for the aerobic oxidation of succinate in liver mitochondria. J Biol Chem 236:1518-1525. |
Azzone GF, Ernster L (1961) J Biol Chem
Abstract: Preincubation of rat liver mitochondria in a sucrose-KCl medium in the presence of 2 to 3 mM arsenate and 0.06 mM Dicumarol or 0.1 mM 2,4dinitrophenol for 3 to 4 minutes results in a marked depression of the succinoxidase capacity. No depression is found when the preincubation is made in the presence of Amytal, cysteine sulfinate, or inorganic phosphate. Addition of adenosine triphosphate after the preincubation stimulates succinate oxidation several-fold. The effect of adenosine triphosphate is not duplicated by cysteine sulfinate, inorganic phosphate, ethylenediaminetetraacetate, adenosine 5’-phosphate, cytidine triphosphate, uridine triphosphate, inosine triphosphate, or guanidine triphosphate. Similar results are obtained with mitochondria pretreated with dinitrophenol and adenosine 5’-phosphate.
The data are consistent with the conclusion that conditions leading to a depletion of the endogenous content of mitochondrial high energy phosphate result in a reversible depression of the succinoxidase capacity.
The concept is developed that the aerobic oxidation of succinate in intact liver mitochondria requires an activation by high energy phosphate. Some implications of this concept regarding the enzymic organization of mitochondrial electron transport and oxidative phosphorylation are discussed. • Keywords: succinate oxidation, high energy phosphate, ATP
Labels:
Organism: Rat
Tissue;cell: Hepatocyte; Liver"Hepatocyte; Liver" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.
Preparation: Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Enzyme: Complex II; Succinate Dehydrogenase"Complex II; Succinate Dehydrogenase" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property.
Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Substrate; Glucose; TCA Cycle"Substrate; Glucose; TCA Cycle" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., ATP; ADP; AMP; PCr"ATP; ADP; AMP; PCr" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
Made history