Baglivo 2022 Abstract Bioblast: Difference between revisions
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Revision as of 12:25, 28 May 2022
Baglivo Eleonora, Cardoso LHD, Cecatto C, Gnaiger E (2022) Statistical analysis of instrumental reproducibility as internal quality control in high-resolution respirometry. Bioblast 2022: BEC Inaugural Conference. ยปMitoFit Preprintยซ |
Link: Bioblast 2022: BEC Inaugural Conference
Baglivo Eleonora, Cardoso Luiza HD, Cecatto Cristiane, Gnaiger Erich (2022)
Event: Bioblast 2022
Evaluation of instrumental reproducibility is a primary component of quality control to quantify the precision and limit of detection of analytical procedures. A pre-analytical instrumental standard operating procedure (SOP) is implemented in high-resolution respirometry consisting of: (1) a daily SOP-POS for air calibration of the polarographic oxygen sensor (POS) in terms of oxygen concentration cO2 [ยตM]. This is part of the sensor test to evaluate POS performance; (2) a monthly SOP-BG starting with the SOP-POS followed by the chamber test quantifying the instrumental O2 background. The chamber test focuses on the slope dcO2/dt [pmolโsโ1โmLโ1] to determine O2 consumption by the POS and O2 backdiffusion into the chamber as a function of cO2 in the absence of sample. Finally, zero O2 calibration completes the sensor test.
We applied this SOP in a 3-year study using 48 Oroboros O2k chambers. Stability of air and zero O2 calibration signals was monitored throughout intervals of up to 8 months without sensor service. Maximum drift over 1 to 3 days was 0.06 pmolโsโ1โmLโ1, without persistence over time since drift was <0.004 pmolโsโ1โmLโ1 for time intervals of one month, corresponding to a drift per day of 0.2 % of the signal at air saturation. Instrumental O2 background -dcO2/dt was stable within ยฑ1 pmolโsโ1โmLโ1 when measured at monthly intervals. These results confirm the instrumental limit of detection of volume-specific O2 flux at ยฑ1 pmolโsโ1โmLโ1. The instrumental SOP applied in the present study contributes to the generally applicable internal quality control management ensuring the unique reproducibility in high-resolution respirometry.
โข Keywords: high-resolution respirometry HRR; polarographic oxygen sensor POS; air calibration; instrumental background; reproducibility; limit of detection; internal quality control IQC; standard operating procedure SOP
โข O2k-Network Lab: AT Innsbruck Oroboros
Affiliations and support
- Oroboros Instruments, Innsbruck, Austria
Figures
List of abbreviations, terms and definitions - MitoPedia
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