Batterson 2023 Physiol Rep

Batterson PM, McGowan EM, Borowik AK, Kinter MT, Miller BF, Newsom SA, Robinson MM (2023) High-fat diet increases electron transfer flavoprotein synthesis and lipid respiration in skeletal muscle during exercise training in female mice. https://doi.org/10.14814/phy2.15840

» Physiol Rep 11:e15840. PMID: 37857571 Open Access

Batterson Philip M, McGowan Erin M, Borowik Agnieszka K, Kinter Michael T, Miller Benjamin F, Newsom Sean A, Robinson Matthew M (2023) Physiol Rep

Abstract: High-fat diet (HFD) and exercise remodel skeletal muscle mitochondria. The electron transfer flavoproteins (ETF) transfer reducing equivalents from β-oxidation into the electron transfer system. Exercise may stimulate the synthesis of ETF proteins to increase lipid respiration. We determined mitochondrial remodeling for lipid respiration through ETF in the context of higher mitochondrial abundance/capacity seen in female mice. We hypothesized HFD would be a greater stimulus than exercise to remodel ETF and lipid pathways through increased protein synthesis alongside increased lipid respiration. Female C57BL/6J mice (n = 15 per group) consumed HFD or low-fat diet (LFD) for 4 weeks then remained sedentary (SED) or completed 8 weeks of treadmill training (EX). We determined mitochondrial lipid respiration, RNA abundance, individual protein synthesis, and abundance for ETFα, ETFβ, and ETF dehydrogenase (ETFDH). HFD increased absolute and relative lipid respiration (p = 0.018 and p = 0.034) and RNA abundance for ETFα (p = 0.026), ETFβ (p = 0.003), and ETFDH (p = 0.0003). HFD increased synthesis for ETFα and ETFDH (p = 0.0007 and p = 0.002). EX increased synthesis of ETFβ and ETFDH (p = 0.008 and p = 0.006). Higher synthesis rates of ETF were not always reflected in greater protein abundance. Greater synthesis of ETF during HFD indicates mitochondrial remodeling which may contribute higher mitochondrial lipid respiration through enhanced ETF function. • Keywords: Exercise, Females, High-fat feeding, Mitochondrial respiration, Skeletal muscle • Bioblast editor: Plangger M • O2k-Network Lab: US CO Colorado Springs Jacobs RA, US OK Oklahoma City Miller BF, US OR Corvallis Robinson MM

Labels: MiParea: Respiration, Exercise physiology;nutrition;life style 

Organism: Mouse 

HRR: Oxygraph-2k 

2023-11