Bioblast alert 2015

Bioblast alert

Bioblast alert messages draw attention to specific topics on the Bioblast wiki.

Bioblast alert - previous issues

Bioblast alert #02 (2011-11-17)

• O2k-Workshop IOC64 - Centre for Cellular and Molecular Biology, Hyderabad, India: 2011-12-08.

• O2k-Publication: Heart failure and mitochondrial dysfunction. Increased OXPHOS capacity after cold preservation of the human heart – a paradox resolved by a dyscoupling mechanism.

Lemieux H, Semsroth S, Antretter H, Hoefer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. Int. J. Biochem. Cell Biol. 43: 1729–1738. -- Discussion: Complementary results on the PGC-1a pathway.

• Scientific service: Tissue storage on ice is possible for limited periods of time (several hours) with preservation of mitochondrial respiratory function, as evaluated with SUIT protocols.

• Term of the week: Uncoupled, non-coupled, dyscoupled - a hallmark of high-resolution respirometry is a matching high-resolution terminology.

Evaluate an example of low-resolution terminology: Uncoupling was studied in stressed cells by measurement of coupled respiration, evaluation of uncoupled respiration after inhibition with oligomycin, and FCCP titration to quantify uncoupled respiration.

Bioblast alert #01 (2011-11-11)

• O2k-Fluorimetry Workshop IOC66: 2012-03-15 to 16. OROBOROS INSTRUMENTS, Innsbruck, Austria. The first series of the O2k-Fluorescence Module will be delivered in April 2012, for orders received by end of January 2012.
• O2k-Publication: A key question on the separation of qualitative versus quantitative change of mitochondrial function is addressed in a new O2k-Publication. Does training induce mild uncoupling? Are sedentary humans healthy controls?

Pesta D, Hoppel F, Macek C, Messner H, Faulhaber M, Kobel C, Parson W, Burtscher M, Schocke M, Gnaiger E (2011) Similar qualitative and quantitative changes of mitochondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans. Am. J. Physiol. Regul. Integr. Comp. Physiol. 301: R1078–R1087.

• Scientific service: ETS capacity plays a key role in such analyses, which cannot be achieved quantitatively without careful uncoupler titrations.
• Term of the month: Basal respiration - a hallmark of high-resolution respirometry is a matching high-resolution terminology.