Bioblast alert 2015

Bioblast alert

Bioblast alert messages draw attention to specific topics on the Bioblast wiki. Bioblast alert is for information purposes only, it is not intended to be an advertisement.

Bioblast alert 2011(02): 2011-11-17

• O2k-Workshop IOC64 - Centre for Cellular and Molecular Biology, Hyderabad, India: 2011-12-08.

• O2k-Publication: Heart failure and mitochondrial dysfunction. Increased OXPHOS capacity after cold preservation of the human heart – a paradox resolved by a dyscoupling mechanism.

Lemieux H, Semsroth S, Antretter H, Hoefer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. Int. J. Biochem. Cell Biol. 43: 1729–1738. >>> Discussion: Complementary results on the PGC-1a pathway.

• Scientific service: Tissue storage on ice is possible for limited periods of time (several hours) with preservation of mitochondrial respiratory function, as evaluated with SUIT protocols.

• Term of the week: Uncoupled, non-coupled, dyscoupled - a hallmark of high-resolution respirometry is a matching high-resolution terminology.

Evaluate an example of low-resolution terminology: Uncoupling was studied in stressed cells by measurement of coupled respiration, evaluation of uncoupled respiration after inhibition with oligomycin, and FCCP titration to quantify uncoupled respiration.

Bioblast alert - previous issues

Bioblast alert 2011(01): 2011-11-11

• O2k-Fluorimetry Workshop IOC66: 2012-03-15 to 16. OROBOROS INSTRUMENTS, Innsbruck, Austria. The first series of the O2k-Fluorescence Module will be delivered in April 2012, for orders received by end of January 2012.
• O2k-Publication: A key question on the separation of qualitative versus quantitative change of mitochondrial function is addressed in a new O2k-Publication. Does training induce mild uncoupling? Are sedentary humans healthy controls?

Pesta D, Hoppel F, Macek C, Messner H, Faulhaber M, Kobel C, Parson W, Burtscher M, Schocke M, Gnaiger E (2011) Similar qualitative and quantitative changes of mitochondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans. Am. J. Physiol. Regul. Integr. Comp. Physiol. 301: R1078–R1087.

• Scientific service: ETS capacity plays a key role in such analyses, which cannot be achieved quantitatively without careful uncoupler titrations.
• Term of the month: Basal respiration - a hallmark of high-resolution respirometry is a matching high-resolution terminology.