Bioblast alert 2015

Bioblast alert is prepared by the Bioblast editorial board to draw attention to specific topics on the Bioblast wiki.

Bioblast alert: for information purposes, not intended to be an advertisement.  
Contributions are welcome.

Bioblast alert 2012(03): 2012-03-23 / 2012-04-03

MitoPedia topic: Oxygen dependence of ROS production - are permeabilized fibres a valid model?

--> Discussion

Drosophila vs. sandworm: High-resolution respirometry in permeabilized muscle fibres

1. Pichaud N, Rioux P, Blier PU (2012) In situ quantification of mitochondrial respiration in permeabilized fibers of a marine invertebrate with low aerobic capacity. Comp Biochem Physiol A Mol Integr Physiol 161: 429-435.
2. Pichaud N, Ballard JWO, Tanguay RM, Blier PU (2011) Thermal sensitivity of mitochondrial functions in permeabilized muscle fibers from two populations of Drosophila simulans with divergent mitotypes. Am J Physiol Regul Integr Comp Physiol 301: R48-R49.

New from the Ballard lab:

• Horan MP, Pichaud N, Ballard JW (2012) Review: Quantifying mitochondrial dysfunction in complex diseases of aging. J Gerontol A Biol Sci Med Sci [Epub ahead of print].

Bioblast alert 2012(02): 2012-03-06

• 50 Years ago: Nick Holonyak, considered as the "father of the light-emitting diode", developed the first practical visible-spectrum LED in 1962. Which colour? What is a LED? >>> see Glossary: Fluorescence

Mitochondrial dys/function in obesity vs. anorexia:

1. Lindfors C, Nilsson IA, Garcia-Roves PM, Zuberi AR, Karimi M, Donahue LR, Roopenian DC, Mulder J, Uhlén M, Ekström TJ, Davisson MT, Hökfelt TG, Schalling M, Johansen JE (2011) Hypothalamic mitochondrial dysfunction associated with anorexia in the anx/anx mouse. Proc Natl Acad Sci U S A 108: 18108-18113.
2. Holmstrom MH, Iglesias-Gutierrez E, Zierath JR, Garcia-Roves PM (2012) Tissue-specific control of mitochondrial respiration in obesity-related insulin resistance and diabetes. Am J Physiol Endocrinol Metab 302: E731-E739.

Bioblast alert 2012(01): 2012-02-27

Two exciting publications on mitochondrial p₅₀, apparent excess capacity of cytochrome c oxidase, basal metabolic rate, P/O ratios and ergometric efficiency:

1. Larsen FJ, Schiffer TA, Sahlin K, Ekblom B, Weitzberg E, Lundberg JO (2011) Mitochondrial oxygen affinity predicts basal metabolic rate in humans. FASEB J 25: 2843-2852.
2. Larsen FJ, Schiffer TA, Borniquel S, Sahlin K, Ekblom B, Lundberg JO, Weitzberg E (2011) Dietary inorganic nitrate improves mitochondrial efficiency in humans. Cell Metab 13: 149-159.

Bioblast alert 2011(04): 2011-12-24

• Congratulations to Dr. Dominik Pesta, for receiving his degree of a PhD at the Medical University of Innsbruck! (Jan 2012)
• Bioblast editorial board - Why a wiki editorial board?
• SUIT protocols for HRR: from intact cells to permeabilized muscle fibres:

Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58.

Bioblast alert 2011(03): 2011-11-24

• Fluorometry combined with high-resolution respirometry: Light guide inserted into the chamber through a black PEEK stopper, or optical probe operated through the O2k-glass window.

• Fluorometry versus fluorimetry - term of the week. >>>New Glossary: Fluorometry - high-resolution terminology.

Bioblast alert 2011(02): 2011-11-17

• O2k-Workshop IOC64 - Centre for Cellular and Molecular Biology, Hyderabad, India: 2011-12-08.

• Heart failure and mitochondrial dysfunction. Increased OXPHOS capacity after cold preservation of the human heart – a paradox resolved by a dyscoupling mechanism.

Lemieux H, Semsroth S, Antretter H, Hoefer D, Gnaiger E (2011) Mitochondrial respiratory control and early defects of oxidative phosphorylation in the failing human heart. Int J Biochem Cell Biol 43: 1729–1738. --> Discussion: Complementary results on the PGC-1a pathway.

• Tissue storage on ice is possible for limited periods of time (several hours) with preservation of mitochondrial respiratory function, as evaluated with SUIT protocols. (Scientific service)

• Uncoupled, non-coupled, dyscoupled - term of the week: a hallmark of high-resolution respirometry is a matching high-resolution terminology.

Evaluate an example of low-resolution terminology: Uncoupling was studied in stressed cells by measurement of coupled respiration, evaluation of uncoupled respiration after inhibition with oligomycin, and FCCP titration to quantify uncoupled respiration.

Bioblast alert 2011(01): 2011-11-11

• A key question on the separation of qualitative versus quantitative change of mitochondrial function is addressed in a new O2k-Publication. Does training induce mild uncoupling? Are sedentary humans healthy controls?

Pesta D, Hoppel F, Macek C, Messner H, Faulhaber M, Kobel C, Parson W, Burtscher M, Schocke M, Gnaiger E (2011) Similar qualitative and quantitative changes of mitochondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans. Am J Physiol Regul Integr Comp Physiol 301: R1078–R1087.

• ETS capacity plays a key role in such analyses, which cannot be achieved quantitatively without careful uncoupler titrations. (Scientific service)
• Basal respiration - term of the month: a hallmark of high-resolution respirometry is a matching high-resolution terminology.