Buck 1993 Am J Physiol Regul Integr Comp Physiol: Difference between revisions
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{{Publication | {{Publication | ||
|title=Buck LT, Hochachka PW, Schön A, Gnaiger E (1993) Microcalorimetric measurement of reversible metabolic suppression induced by anoxia in isolated hepatocytes. Am J Physiol Regul Integr Comp Physiol 265: R1014- | |title=Buck LT, Hochachka PW, Schön A, Gnaiger E (1993) Microcalorimetric measurement of reversible metabolic suppression induced by anoxia in isolated hepatocytes. Am J Physiol Regul Integr Comp Physiol 265:R1014-9. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/8238601 PMID: 8238601] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/8238601 PMID: 8238601] | ||
|authors=Buck LT, Hochachka PW, Schoen A, Gnaiger | |authors=Buck LT, Hochachka PW, Schoen A, Gnaiger Erich | ||
|year=1993 | |year=1993 | ||
|journal=Am J Physiol Regul Integr Comp Physiol | |journal=Am J Physiol Regul Integr Comp Physiol | ||
|abstract=The metabolic suppression due to anoxia in hepatocytes from the anoxia-tolerant turtle ''Chrysemys picta bellii'' was measured directly using microcalorimetric techniques. The normoxic heat flux from hepatocytes in suspension (25 °C) was 1.08 +/- 0.08 mW/g cells and decreased by 76% to 0.26 +/- 0.03 mW/g cells in response to anoxic incubation. After an acute decrease in temperature (to 10 °C) anoxic heat flux dropped by 96% relative to the normoxic control at 25 degrees C. The relative decrease in heat flux at both temperatures was similar, 76% at 25 °C and 68% at 10 °C. From the caloric equivalent of glycogen fermentation to lactate the heat flux from lactate production was calculated to be -93 µW/g cells (25 °C), and this accounted for 36% of the anoxic heat flux. When the enthalpy change associated with the release of free glucose (from glycogen breakdown) is considered, an additional 6% of the anoxic heat flux can be accounted for. Therefore, a portion of the anoxic heat flux is unaccounted for (58%), resulting in an "exothermic gap." This differs from the normoxically incubated hepatocytes where the indirect calorimetric measurement of heat flux (hepatocyte O<sub>2</sub> consumption) could fully account for the calorimetrically measured heat flux. When normoxic hepatocytes were inhibited with cyanide, a rapid suppression in heat flux was observed. Because rapid reequilibration to a lower, cyanide-induced steady state occurred in < 15 min, it is also assumed that there is no short-term Pasteur effect in this tissue. (ABSTRACT TRUNCATED AT 250 WORDS) | |abstract=The metabolic suppression due to anoxia in hepatocytes from the anoxia-tolerant turtle ''Chrysemys picta bellii'' was measured directly using microcalorimetric techniques. The normoxic heat flux from hepatocytes in suspension (25 °C) was 1.08 +/- 0.08 mW/g cells and decreased by 76% to 0.26 +/- 0.03 mW/g cells in response to anoxic incubation. After an acute decrease in temperature (to 10 °C) anoxic heat flux dropped by 96% relative to the normoxic control at 25 degrees C. The relative decrease in heat flux at both temperatures was similar, 76% at 25 °C and 68% at 10 °C. From the caloric equivalent of glycogen fermentation to lactate the heat flux from lactate production was calculated to be -93 µW/g cells (25 °C), and this accounted for 36% of the anoxic heat flux. When the enthalpy change associated with the release of free glucose (from glycogen breakdown) is considered, an additional 6% of the anoxic heat flux can be accounted for. Therefore, a portion of the anoxic heat flux is unaccounted for (58%), resulting in an "exothermic gap." This differs from the normoxically incubated hepatocytes where the indirect calorimetric measurement of heat flux (hepatocyte O<sub>2</sub> consumption) could fully account for the calorimetrically measured heat flux. When normoxic hepatocytes were inhibited with cyanide, a rapid suppression in heat flux was observed. Because rapid reequilibration to a lower, cyanide-induced steady state occurred in < 15 min, it is also assumed that there is no short-term Pasteur effect in this tissue. (ABSTRACT TRUNCATED AT 250 WORDS) | ||
|keywords=Direct and Indirect Calorimetry, Enthalpy, Pasteur effect, Chrysemys picta bellii turtle | |keywords=Direct and Indirect Calorimetry, Enthalpy, Pasteur effect, ''Chrysemys picta bellii'' turtle | ||
|mipnetlab= | |mipnetlab=AT Innsbruck Gnaiger E | ||
}} | }} | ||
== Cited by == | |||
::* 10 articles in PubMed (2021-12-27) https://pubmed.ncbi.nlm.nih.gov/8238601/ | |||
{{Labeling | {{Labeling | ||
| | |area=Respiration, Comparative MiP;environmental MiP | ||
|injuries= | |injuries=Ischemia-reperfusion | ||
| | |organism=Reptiles | ||
|tissues=Liver | |tissues=Liver | ||
|preparations=Intact | |preparations=Intact cells | ||
|couplingstates=LEAK, ROUTINE | |couplingstates=LEAK, ROUTINE | ||
| | |instruments=Oxygraph-2k | ||
| | |additional=CaloRespirometry | ||
}} | }} | ||
Latest revision as of 14:37, 27 December 2021
» [[Has info::PMID: 8238601]]
Was written by::Buck LT, Was written by::Hochachka PW, Was written by::Schoen A, Was written by::Gnaiger Erich (Was published in year::1993) Was published in journal::Am J Physiol Regul Integr Comp Physiol
Abstract: [[has abstract::The metabolic suppression due to anoxia in hepatocytes from the anoxia-tolerant turtle Chrysemys picta bellii was measured directly using microcalorimetric techniques. The normoxic heat flux from hepatocytes in suspension (25 °C) was 1.08 +/- 0.08 mW/g cells and decreased by 76% to 0.26 +/- 0.03 mW/g cells in response to anoxic incubation. After an acute decrease in temperature (to 10 °C) anoxic heat flux dropped by 96% relative to the normoxic control at 25 degrees C. The relative decrease in heat flux at both temperatures was similar, 76% at 25 °C and 68% at 10 °C. From the caloric equivalent of glycogen fermentation to lactate the heat flux from lactate production was calculated to be -93 µW/g cells (25 °C), and this accounted for 36% of the anoxic heat flux. When the enthalpy change associated with the release of free glucose (from glycogen breakdown) is considered, an additional 6% of the anoxic heat flux can be accounted for. Therefore, a portion of the anoxic heat flux is unaccounted for (58%), resulting in an "exothermic gap." This differs from the normoxically incubated hepatocytes where the indirect calorimetric measurement of heat flux (hepatocyte O2 consumption) could fully account for the calorimetrically measured heat flux. When normoxic hepatocytes were inhibited with cyanide, a rapid suppression in heat flux was observed. Because rapid reequilibration to a lower, cyanide-induced steady state occurred in < 15 min, it is also assumed that there is no short-term Pasteur effect in this tissue. (ABSTRACT TRUNCATED AT 250 WORDS)]] • Keywords: has publicationkeywords::Direct and Indirect Calorimetry, has publicationkeywords::Enthalpy, has publicationkeywords::Pasteur effect, has publicationkeywords::''Chrysemys picta bellii'' turtle
• O2k-Network Lab: Was published by MiPNetLab::AT Innsbruck Gnaiger E
Cited by
- 10 articles in PubMed (2021-12-27) https://pubmed.ncbi.nlm.nih.gov/8238601/
Labels: MiParea: MiP area::Respiration, MiP area::Comparative MiP;environmental MiP
Stress:Injury and adaptation::Ischemia-reperfusion Organism: Organism::Reptiles Tissue;cell: tissue and cell::Liver Preparation: Preparation::Intact cells
Coupling state: Coupling states::LEAK, Coupling states::ROUTINE
