Burtscher 2015 Mitochondrion: Difference between revisions
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{{Publication | {{Publication | ||
|title=Burtscher J, Zangrandi L, Schwarzer C, Gnaiger E (2015) Differences in mitochondrial function in homogenated samples from healthy and epileptic specific brain tissues revealed by high-resolution respirometry. Mitochondrion doi:10.1016/j.mito.2015.10.007. | |title=Burtscher J, Zangrandi L, Schwarzer C, Gnaiger E (2015) Differences in mitochondrial function in homogenated samples from healthy and epileptic specific brain tissues revealed by high-resolution respirometry. Mitochondrion doi:10.1016/j.mito.2015.10.007. | ||
|info=[http://www. | |info=[http://www.ncbi.nlm.nih.gov/pubmed/26516105 PMID: 26516105 Open Access] | ||
|authors=Burtscher J, Zangrandi L, Schwarzer C, Gnaiger E | |authors=Burtscher J, Zangrandi L, Schwarzer C, Gnaiger E | ||
|year=2015 | |year=2015 | ||
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In summary, respirometric OXPHOS analysis provides a very sensitive diagnostic approach using small amounts of distinct brain tissues. In a single assay, information is obtained on numerous OXPHOS parameters as indicators of tissue-specific mitochondrial performance. | In summary, respirometric OXPHOS analysis provides a very sensitive diagnostic approach using small amounts of distinct brain tissues. In a single assay, information is obtained on numerous OXPHOS parameters as indicators of tissue-specific mitochondrial performance. | ||
|keywords=Neurodegeneration, Epilepsy, Mitochondria, Brain regions, Respirometry | |keywords=Neurodegeneration, Epilepsy, Mitochondria, Brain regions, Respirometry | ||
|mipnetlab=AT Innsbruck Gnaiger E, AT Innsbruck OROBOROS | |mipnetlab=AT Innsbruck Gnaiger E, AT Innsbruck OROBOROS | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
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|couplingstates=LEAK, OXPHOS, ETS | |couplingstates=LEAK, OXPHOS, ETS | ||
|substratestates=CI, CII, CIV, CI&II, ROX | |substratestates=CI, CII, CIV, CI&II, ROX | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional=PBI - Shredder | |additional=PBI - Shredder | ||
}} | }} |
Revision as of 10:59, 10 November 2015
Burtscher J, Zangrandi L, Schwarzer C, Gnaiger E (2015) Differences in mitochondrial function in homogenated samples from healthy and epileptic specific brain tissues revealed by high-resolution respirometry. Mitochondrion doi:10.1016/j.mito.2015.10.007. |
Burtscher J, Zangrandi L, Schwarzer C, Gnaiger E (2015) Mitochondrion
Abstract: Mitochondrial dysfunction and oxidative stress are strongly implicated in neurodegenerative diseases and epilepsy. Strikingly, neurodegenerative diseases show regional specificity in vulnerability and follow distinct patterns of neuronal loss. A challenge is to understand, why mitochondria fail in particular brain regions under specific pathological conditions. A potential explanation could be provided by regional or cellular specificity of mitochondrial function.
We applied high-resolution respirometry to analyze the integrated Complex I- and II (CI and CII)-linked respiration, the activity of Complex IV, and the combined CI&II-linked oxidative phosphorylation (OXPHOS)- and electron-transfer system (ETS)-capacity in microsamples obtained from distinct regions of the mouse brain. We compared different approaches to assess mitochondrial density and suggest flux control ratios as a valid method to normalize respiration to mitochondrial density.
This approach revealed significant differences of CI- and CII-linked OXPHOS capacity and coupling control between motor cortex, striatum, hippocampus and pons of naΓ―ve mice. CI-linked respiration was highest in motor cortex, while CII-linked respiration predominated in the striatum. To investigate if this method could also determine differences in normal and disease states within the same brain region, we compared hippocampal homogenates in a chronic epilepsy model. Three weeks after stereotaxic injection of kainate, there was a down-regulation of CI- and upregulation of CII-linked respiration in the resulting epileptic ipsilateral hippocampus compared to the contralateral one.
In summary, respirometric OXPHOS analysis provides a very sensitive diagnostic approach using small amounts of distinct brain tissues. In a single assay, information is obtained on numerous OXPHOS parameters as indicators of tissue-specific mitochondrial performance. β’ Keywords: Neurodegeneration, Epilepsy, Mitochondria, Brain regions, Respirometry
β’ O2k-Network Lab: AT Innsbruck Gnaiger E, AT Innsbruck OROBOROS
Labels: MiParea: Respiration, Instruments;methods, mt-Biogenesis;mt-density, Pharmacology;toxicology
Pathology: Neurodegenerative
Stress:Mitochondrial disease
Organism: Mouse
Tissue;cell: Nervous system
Preparation: Permeabilized tissue, Homogenate
Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k
PBI - Shredder