Difference between revisions of "Calcium Green"
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* Ca2+ calibration buffers. | * Ca2+ calibration buffers. | ||
{{Template:Technical support integrated}} | |||
=== Ca measurement === | |||
See [[O2k-Fluo LED2-Module]] for specific aspects of fluorescence measurements using the O2k. | See [[O2k-Fluo LED2-Module]] for specific aspects of fluorescence measurements using the O2k. | ||
On the fluorophores producers homepage detailed product information ([https://tools.lifetechnologies.com/content/sfs/manuals/mp03010.pdf | Long-Wavelength Calcium Indicators Product Information ], [http://tools.lifetechnologies.com/content/sfs/manuals/CalciumCalibrationBufferKits_PI.pdf |CalciumCalibration]) as well as general information on measuring calcium (e.g. [http://www.lifetechnologies.com/at/en/home/references/molecular-probes-the-handbook/indicators-for-ca2-mg2-zn2-and-other-metal-ions/introduction-to-ca2-measurements-with-fluorescent-indicators.html], [http://www.lifetechnologies.com/at/en/home/references/molecular-probes-the-handbook/indicators-for-ca2-mg2-zn2-and-other-metal-ions/fluorescent-ca2-indicators-excited-with-visible-light.html]) is available. | On the fluorophores producers homepage detailed product information ([https://tools.lifetechnologies.com/content/sfs/manuals/mp03010.pdf | Long-Wavelength Calcium Indicators Product Information ], [http://tools.lifetechnologies.com/content/sfs/manuals/CalciumCalibrationBufferKits_PI.pdf |CalciumCalibration]) as well as general information on measuring calcium (e.g. [http://www.lifetechnologies.com/at/en/home/references/molecular-probes-the-handbook/indicators-for-ca2-mg2-zn2-and-other-metal-ions/introduction-to-ca2-measurements-with-fluorescent-indicators.html], [http://www.lifetechnologies.com/at/en/home/references/molecular-probes-the-handbook/indicators-for-ca2-mg2-zn2-and-other-metal-ions/fluorescent-ca2-indicators-excited-with-visible-light.html]) is available. | ||
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== Signal and output == | === Signal and output === | ||
# Signal: The [[O2k-Fluo LED2-Module]] is operated through the amperometric (Amp)-Channel of the O2k, with electric current (ampere | # Signal: The [[O2k-Fluo LED2-Module]] is operated through the amperometric (Amp)-Channel of the O2k, with electric current (ampere [Amp]) as the primary signal. | ||
# Output: The focus of the output with Calcium Green is on [[O2k signal and output|'''Type A''']]: Concentration, and [[O2k | # Output: The focus of the output with Calcium Green is on [[O2k signal and output|'''Type A''']]: Concentration, and [[O2k signals and output|'''Type C''']]: Force, potential. | ||
=== References === | |||
* [[Elustondo 2014 Biochim Biophys Acta]] - Use of Calcium Green-5N with the [[O2k-Fluorescence LED2-Module]]. | |||
* Use of Calcium Green-5N with the [[O2k-Fluorescence LED2-Module]] | |||
* [[Anderson_2011_Am_J_Physiol_Heart_Circ_Physiol]] | * [[Anderson_2011_Am_J_Physiol_Heart_Circ_Physiol]] | ||
Revision as of 10:28, 6 February 2016
- high-resolution terminology - matching measurements at high-resolution
Calcium Green
Description
Calcium Green denotes a family of extrinsic fluorophores applied for measurement of Ca2+ concentration.
Abbreviation: CaG
MitoPedia methods:
Fluorometry
Calcium GreenTM is a registered trademark and available from Life Technologies (former Invitrogen) in several formulations.
All fluorophores of the Calcium Green family have the same fluorophoric moiety in their molecular structure and therefore near identical optical properties. They differ in their KD values and are suitable for measuring different ranges of calcium concentrations.
- Calcium Green 1: KD = 190 nM
- Calcium Green-2: KD = 550 nM
- Calcium Green-5N: KD = 14000 nM
Measurement: See Calcium for method independent considerations related to measuring calcium levels, covering e.g.
- important consideration before starting a project involving calcium measurements
- Ca2+ buffering
- calculation of free Ca2+ concentrations
- Ca2+ calibration buffers.
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
Ca measurement
See O2k-Fluo LED2-Module for specific aspects of fluorescence measurements using the O2k. On the fluorophores producers homepage detailed product information (| Long-Wavelength Calcium Indicators Product Information , |CalciumCalibration) as well as general information on measuring calcium (e.g. [1], [2]) is available.
The three Calcium Green fluorophores and Magnesium Green share the same fluorophore moiety in their molecular structure, therefore the optical properties are very similar and the same fluorescence sensor and filter set can be used. Since the used concentration of Calcium Green might vary according to application, the optimum light intensity and amplification used may be different to those for Magnesium Green. However, the values recommended for Magnesium Green will provide a good starting point.
Signal and output
- Signal: The O2k-Fluo LED2-Module is operated through the amperometric (Amp)-Channel of the O2k, with electric current (ampere [Amp]) as the primary signal.
- Output: The focus of the output with Calcium Green is on Type A: Concentration, and Type C: Force, potential.
References
- Elustondo 2014 Biochim Biophys Acta - Use of Calcium Green-5N with the O2k-Fluorescence LED2-Module.
- Anderson_2011_Am_J_Physiol_Heart_Circ_Physiol
