Difference between revisions of "Calcium Green"

Revision as of 11:22, 6 June 2012

high-resolution terminology - matching measurements at high-resolution

Calcium Green

Description

Calcium green denotes a family of extrinsic fluorophores applied for measurement of Ca²⁺ concentration.

Abbreviation: CaG

MitoPedia methods: Fluorometry

Calcium green^TM is a registered trademark and available from Invitrogen in several formulations. All fluorophores of the Calcium green family have the same fluorophoric moiety in their molecular structure and therefore near identical optical properties. They differ in their K_D values and are suitable for measuring different ranges of calcium concentrations.

Calcium green 1: K_D = 190 nM
Calcium green-2: K_D = 550 nM
Calcium green-5N: K_D = 14000 nM

Measurement: see O2k-Fluorescence LED2-Module The three Calcium green fluorophores and Magnesium green share the same fluorophore moiety in their molecular structure, therefore the optical properties are very similar and the same fluorescence sensor and filter set can be used. Since the used concentration of Calcium green might vary according to application, the optimum light intensity and amplification used may be different to those for Magnesium green. However, the values recommended for Magnesium green will provide a good starting point.

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 {{MitoPedia
 |abbr=CaG
-|description='''Calcium green''' belongs to the [[extrinsic fluorophores]] applied for measurement of Ca<sup>2+</sup> concentration (K<sub>D</sub> = 190 nM.
+|description='''Calcium green''' denotes a family of [[extrinsic fluorophores]] applied for measurement of Ca<sup>2+</sup> concentration.
 }}
 {{MitoPedia methods
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 }}
 {{MitoPedia topics}}
+Calcium green<sup>TM</sup> is a registered trademark and available from Invitrogen in several formulations.
+All fluorophores of the Calcium green family have the same fluorophoric moiety in their molecular structure and therefore  near identical optical properties. They differ in their  K<sub>D</sub> values and are suitable for measuring  different ranges of calcium concentrations.
+* Calcium green 1: K<sub>D</sub> = 190 nM
+* Calcium green-2: K<sub>D</sub> = 550 nM
+* Calcium green-5N: K<sub>D</sub> = 14000 nM
-Calcium green<sup>TM</sup> is a registered trademark and available from Invitrogen in several formulations.
 '''Measurement:''' see [[O2k-Fluorescence LED2-Module]]
-Calcium green and [[Magnesium green]] share the same fluorophore moiety in their molecular structure, therefore the optical properties are very similar and the same [[Fluorescence-Sensor Blue|fluorescence sensor]] and [[Filter Set MgG / CaG|filter set]] can be used. Since the used concentration of Calcium green might vary according to application, the optimum light intensity and amplification used may be different to those for Magnesium green. However, the values recommended for Magnesium green will provide a good starting point.
+The three Calcium green fluorophores and [[Magnesium green]] share the same fluorophore moiety in their molecular structure, therefore the optical properties are very similar and the same [[Fluorescence-Sensor Blue|fluorescence sensor]] and [[Filter Set MgG / CaG|filter set]] can be used. Since the used concentration of Calcium green might vary according to application, the optimum light intensity and amplification used may be different to those for Magnesium green. However, the values recommended for Magnesium green will provide a good starting point.