- high-resolution terminology - matching measurements at high-resolution
Calcium Green
Description
Calcium green denotes a family of extrinsic fluorophores applied for measurement of Ca2+ concentration.
Abbreviation: CaG
MitoPedia methods:
Fluorometry
Calcium greenTM is a registered trademark and available from Invitrogen in several formulations.
All fluorophores of the Calcium green family have the same fluorophoric moiety in their molecular structure and therefore near identical optical properties. They differ in their KD values and are suitable for measuring different ranges of calcium concentrations.
- Calcium green 1: KD = 190 nM
- Calcium green-2: KD = 550 nM
- Calcium green-5N: KD = 14000 nM
Measurement: see O2k-Fluorescence LED2-Module
The three Calcium green fluorophores and Magnesium green share the same fluorophore moiety in their molecular structure, therefore the optical properties are very similar and the same fluorescence sensor and filter set can be used. Since the used concentration of Calcium green might vary according to application, the optimum light intensity and amplification used may be different to those for Magnesium green. However, the values recommended for Magnesium green will provide a good starting point.
