- high-resolution terminology - matching measurements at high-resolution
Carbonyl cyanide m-chlorophenyl hydrazone
Description
Carbonyl cyanide m-chloro phenyl hydrazone (C9H5ClN4; FW = 204.62) is a chemical inhibitor of oxidative phosphorylation and causes an uncoupling (uncoupler) of the proton gradient that is established during the normal activity of electron carriers in the electron transport system.
Abbreviation: CCCP
MitoPedia topics:
Uncoupler
Labels:
Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
HRR: Chemicals; Media"Chemicals; Media" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
Application in HRR
C: CCCP (Carbonyl cyanide 3-chlorophenylhydrazone , C9H5ClN4O); Sigma C 2759, 100 mg, store at -20 °C: FW = 204.62)
Caution: Toxic!
Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
Preparation of 1 mM stock solution (dissolved in DMSO):
- 1) weigh 1.02 mg of CCCP into a glass vial
- 2) dissolve in 5 ml DMSO
- 3) divide into 0.2 ml portions OR store in 5 ml glass vial and use subsamples during experiments to avoid contamination of the stock solution
- 3) store in glass vials at -20 °C
