Difference between revisions of "Carnitine"
From Bioblast
(replaced FW by M and added units g*mol-1) |
(added instructions for small-volume-chamber) |
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
::::* Car: '''L-carnitine:''' Sigma Aldrich; C0158; M = 161.2 g·mol<sup>-1</sup> | ::::* Car: '''L-carnitine:''' Sigma Aldrich; C0158; M = 161.2 g·mol<sup>-1</sup> | ||
::::'''Preparation of 250 mM stock solution (dissolved in H<sub>2</sub>O) | |||
:::: '''Preparation of 250 mM stock solution''' (dissolved in H<sub>2</sub>O) | |||
::::# Weigh 100 mg of Carnitine. | ::::# Weigh 100 mg of Carnitine. | ||
::::# Add 2.48 mL H<sub>2</sub>O. | ::::# Add 2.48 mL H<sub>2</sub>O. | ||
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::::# Store frozen at -20°C. | ::::# Store frozen at -20°C. | ||
::: | :::» '''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | ||
::::* Titration volume: 4 µL | |||
::::* Titration volume ('''2-mL O2k-chamber'''): 4 µL using a 10 µL Hamilton syringe. | |||
::::* Titration volume ('''0.5-mL O2k-chamber'''): 1 µL using a 10 µL Hamilton syringe | |||
::::* Final concentration: 0.5 mM. | ::::* Final concentration: 0.5 mM. | ||
Revision as of 10:48, 22 March 2021
Description
Carnitine is an important factor for the transport of long-chain fatty acids bound to carnitine (carnitine acyltransferase) into the mitochondrial matrix for subsequent β-oxidation. There are two enantiomers: D- and L-carnitine. Only the L-isomer is physiologically active.
Abbreviation: Car
Application in HRR
- Car: L-carnitine: Sigma Aldrich; C0158; M = 161.2 g·mol-1
- Preparation of 250 mM stock solution (dissolved in H2O)
- Weigh 100 mg of Carnitine.
- Add 2.48 mL H2O.
- Divide into 100 µL portions.
- Store frozen at -20°C.
- » O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 4 µL using a 10 µL Hamilton syringe.
- Titration volume (0.5-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe
- Final concentration: 0.5 mM.
Feeding carnitine
MitoPedia topics:
Substrate and metabolite