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Difference between revisions of "Carrier control titrations"

From Bioblast
(Created page with "When performing an experiment is necessary to do a control with the carrier of your drugs to test for secondary effects over the bioenergetics parameters.")
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When performing an experiment is necessary to do a control with the carrier of your drugs to test for secondary effects over the bioenergetics parameters.
Most of the nonpolar compounds have to be diluted in organic solvents such as DMSO or acetonitrile in order to use them for the titrations in the SUIT protocols. However, the solvent (carrier) itself could affect the mitochondrial physiology and promote alterations that we need to take into account. For this reason, it is necessary to run in parallel to our treatment experiment a control experiment on which we will add a carrier titration to test if it affects our sample or not.

Revision as of 16:22, 10 April 2019

Most of the nonpolar compounds have to be diluted in organic solvents such as DMSO or acetonitrile in order to use them for the titrations in the SUIT protocols. However, the solvent (carrier) itself could affect the mitochondrial physiology and promote alterations that we need to take into account. For this reason, it is necessary to run in parallel to our treatment experiment a control experiment on which we will add a carrier titration to test if it affects our sample or not.