Difference between revisions of "Carrier control titrations"
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Most of the nonpolar compounds have to be diluted in organic solvents such as DMSO or acetonitrile in order to use them for the titrations in the SUIT protocols. However, the solvent (carrier) itself could affect the mitochondrial physiology and promote alterations that we need to take into account. For this reason, it is necessary to run in parallel to our treatment experiment a control experiment on which we will add a carrier titration to test if it affects our sample or not. | {{MitoPedia | ||
|description=Most of the nonpolar compounds have to be diluted in organic solvents such as DMSO or acetonitrile in order to use them for the titrations in the SUIT protocols. However, the solvent (carrier) itself could affect the mitochondrial physiology and promote alterations that we need to take into account. For this reason, it is necessary to run in parallel to our treatment experiment a control experiment on which we will add a '''carrier control titration''' to test if it affects our sample or not. | |||
{{MitoPedia | |info=[[Hochachka 2002 Oxford Univ Press | Hochachka, Somero 2002]] | ||
|mitopedia | }} | ||
{{MitoPedia concepts | |||
|mitopedia concept=MiP concept | |||
}} | }} | ||
Revision as of 16:28, 10 April 2019
- high-resolution terminology - matching measurements at high-resolution
Carrier control titrations
Description
Most of the nonpolar compounds have to be diluted in organic solvents such as DMSO or acetonitrile in order to use them for the titrations in the SUIT protocols. However, the solvent (carrier) itself could affect the mitochondrial physiology and promote alterations that we need to take into account. For this reason, it is necessary to run in parallel to our treatment experiment a control experiment on which we will add a carrier control titration to test if it affects our sample or not.
Reference: Hochachka, Somero 2002
MitoPedia concepts:
MiP concept
