Cervinkova 2012 Abstract Bioblast
| Cervinkova Z (2012) Evaluation of mitochondrial functions – an important tool for experimental hepatology Mitochondr Physiol Network 17.12. |
Link: MiPNet17.12 Bioblast 2012 - Open Access
Cervinkova Z, Stanková P, Cervinka M, Kucera O, Drahota Z (2012)
Event: Bioblast 2012
Liver plays an essential role in intermediary and energy metabolism; besides other important functions hepatocytes are responsible for biotransformation of the majority of endogenous and exogenous substances including most of drugs. The majority of toxic agents act either fully or partially via oxidative stress, the liver, specifically the mitochondria in hepatocytes, being the main target. Maintenance of mitochondrial function is essential for the survival and normal performance of hepatocytes, which have a high energy requirement. Liver has amazing capacity to regenerate after toxic liver injury or partial hepatectomy; resulting proliferative processes represent a great demand for energy. Therefore, understanding of the role of mitochondria in hepatocytes is of fundamental importance. Our research group has been working for many years in the field of experimental hepatology, namely in: 1. Liver regeneration induced by partial hepatectomy or toxic injury in vivo 2. Study of the mechanisms responsible for toxic liver injury in vivo and in vitro 3. Study of liver regeneration and toxic injury in the terrain of NAFLD (non-alcoholic fatty liver disease) which became the most common chronic disease of the liver. In our projects we analyse mitochondrial function using several basic models: hepatocytes cultured in vitro; mitochondria in permeabilised hepatocytes; tissue homogenates; and isolated mitochondria. For measurement of oxygen uptake we are using High Resolution Oxygraph 2K, for evaluation of mitochondrial membrane potential (MMP) tetraphenylphosphonium (TPP+) electrode or accumulation of Rhodamin123; JC-1 a fluorescence probe is used for visualisation of the MMP. Using these methods we contributed to the knowledge about role and changes in liver mitochondria under various pathological conditions. There is another important outcome of our work. As a university department an essential part of our activities is education. We have participated on two European projects within last 3 years; HEPIN - Hepatology Institute in Hradec Kralove and PhysiSciNet (Physiology Science Network). In frame of these projects we have organised twice a year Bioenergetic Workshops, so we became a training base for young researchers from the Czech Republic being interested in bioenergetics.
• Keywords: hepatocytes, high-resolution respirometry, mitochondria, mitochondrial membrane potential
• O2k-Network Lab: CZ Hradec Kralove Cervinkova Z
Labels:
Stress:RONS; Oxidative Stress"RONS; Oxidative Stress" is not in the list (Cell death, Cryopreservation, Ischemia-reperfusion, Permeability transition, Oxidative stress;RONS, Temperature, Hypoxia, Mitochondrial disease) of allowed values for the "Stress" property. Organism: Rat Tissue;cell: Hepatocyte; Liver"Hepatocyte; Liver" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property. Preparation: Intact Cell; Cultured; Primary"Intact Cell; Cultured; Primary" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property., Permeabilized cells, Homogenate, Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property. Enzyme: Complex I, Complex II; Succinate Dehydrogenase"Complex II; Succinate Dehydrogenase" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property. Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Membrane Potential"Membrane Potential" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property. Coupling state: OXPHOS
HRR: Oxygraph-2k, Spectrofluorimetry"Spectrofluorimetry" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
Affiliations and author contributions
Cervinkova Z (1), Stanková P (1), Cervinka M (2), Kucera O (1), Drahota Z(1) (1) Department of Physiology (2) Department of Medical Biology and Genetics Charles University in Prague, Faculty of Medicine in Hradec Kralove, Simkova 870, 500 38 Hradec Kralove, Czech Republic
