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== Affiliations ==
::::Chabi Béatrice(1), Ost M(2), Gama-Perez P(3), Dahdah N(3), Lemieux H(4), Holody CD(4), Carpenter RG(4), Tepp K(5), Puurand M(5), Kaambre T(5), Dubouchaud H(6), Cortade F(1), Pesta D(7,8), Calabria E(9), Casado M(10), Fernandez-Ortiz M(11), Acuña-Castroviejo D(11), Villena JA(12), Grefte S(13), Keijer J(13), O'Brien K(14), Sowton A(14), Murray AJ(14), Campbell MD(15), Marcinek DJ(15), Nollet E(16), Wüst R(16), Dayanidhi S(17), Gnaiger E(18,19), Doerrier C(18), Garcia-Roves PM(3)
::::#DMEM, INRA, Univ Montpellier, France
::::#German Inst Human Nutrition Potsdam-Rehbruecke, Potsdam, Germany
::::#Dept Physiological Sciences, Univ Barcelona Bellvitge Biomedical Research Inst (IDIBELL), Spain
::::#Fac Saint-Jean, Univ Alberta, Canada
::::#Lab Chemical Biology, National Inst Chemical Physics Biophysics, Tallinn, Estonia
::::#Lab Bioénergétique Fondamentale Appliquée, Univ Grenoble Alpes, INSERM, Grenoble, France
::::#Inst Clinical Diabetology, German Diabetes Center, Leibniz Center Diabetes Research Heinrich-Heine Univ Düsseldorf, Germany
::::#German Center Diabetes Research, Munich, Neuherberg, Germany
::::#Dept Neurological Movement Sciences, Univ Verona, Italy
::::#Dept Molecular Cellular Pathology Therapy, Inst Biomedicina de Valencia, Spain
::::#Biomedical Research Center, Univ Granada, Spain
::::#Metabolism Obesity Lab, Vall d’Hebron Research Inst, Barcelona, Spain
::::#Human Animal Physiology, Wageningen Univ, The Netherlands
::::#Dept Physiology, Development Neuroscience, Univ Cambridge, UK
::::#Dept Radiology, Univ Washington, South Lake Union, USA
::::#Dept Human Movement Sciences, Fac Behavioural Movement Sciences, Vrije Univ Amsterdam, The Netherlands
::::#Rehabilitation Inst Chicago, Feinberg School Medicine, Northwestern Univ, USA
::::#Oroboros Instruments, Innsbruck, Austria
::::#Dept Visceral, Transplant Thoracic Surgery, Daniel Swarovski Research Lab, Medical Univ Innsbruck, Austria
== Figures ==
[[File:Chabi_Figure1_MiP2019.jpg|left|400 px]] Figure 1. Substrate-uncoupler-inhibitor titration protocol (SUIT-008 O2 pfi D014). Sequential titrations and respiratory states. 1PM: NADH-pathway (N-pathway) in the presence of 5 mM pyruvate and 2 mM malate in the N-LEAK state. 2D: saturating ADP (N-OXPHOS state). 2c: 10 μM cytochrome c for evaluating the integrity of the outer mitochondrial membrane. 3G: 10 mM glutamate as an additional NADH-linked substrate (N-OXPHOS state). 4S: 10 mM succinate (NS-OXPHOS capacity). 5U: uncoupler titrations to evaluate the electron transfer- (ET-) capacity (NS-ET capacity). 6Rot: inhibition of CI by rotenone (S-ET capacity). 7Ama: inhibition of CIII by antimycin A (residual oxygen consumption, Rox). Oxygen concentration range in the experiment was maintained between 400-250 µM O2.
[[File:Chabi_Figure2_MiP2019.jpg|left|400 px]] Figure 2. NS-OXPHOS capacity of permeabilized soleus muscle fibers. NS-OXPHOS capacity was measured in permeabilized soleus muscle fibers from male C57BL/6J mice by 16 research groups. Median with interquartile range show results from individual group with muscle fibers obtained from at least four soleus muscles.
== References ==
::::#Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840. [[SUIT-8_O2_pfi_D14]]
::::#Baker M (2016) 1,500 scientists lift the lid on reproducibility. Survey sheds light on the ‘crisis’ rocking research. Nature 533:452–4.
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