Difference between revisions of "Chowdhury 2010 Diabetes"

Latest revision as of 19:10, 1 April 2018

Chowdhury SK, Zherebitskaya E, Smith DR, Akude E, Chattopadhyay S, Jolivalt CG, Calcutt NA, Fernyhough P (2010) Mitochondrial respiratory chain dysfunction in dorsal root ganglia of streptozotocin-induced diabetic rats and its correction by insulin treatment. Diabetes 59:1082-91.

» PMID: 20103706 Open Access

Chowdhury SK, Zherebitskaya E, Smith DR, Akude E, Chattopadhyay S, Jolivalt CG, Calcutt NA, Fernyhough P (2010) Diabetes

Abstract: Objective: Impairments in mitochondrial physiology may play a role in diabetic sensory neuropathy. We tested the hypothesis that mitochondrial dysfunction in sensory neurons is due to abnormal mitochondrial respiratory function.

Research design and methods: Rates of oxygen consumption were measured in mitochondria from dorsal root ganglia (DRG) of 12- to- 22-week streptozotocin (STZ)-induced diabetic rats, diabetic rats treated with insulin, and age-matched controls. Activities and expression of components of mitochondrial complexes and reactive oxygen species (ROS) were analyzed.

Results: Rates of coupled respiration with pyruvate + malate (P + M) and with ascorbate + TMPD (Asc + TMPD) in DRG were unchanged after 12 weeks of diabetes. By 22 weeks of diabetes, respiration with P + M was significantly decreased by 31-44% and with Asc + TMPD by 29-39% compared with control. Attenuated mitochondrial respiratory activity of STZ-diabetic rats was significantly improved by insulin that did not correct other indices of diabetes. Activities of mitochondrial complexes I and IV and the Krebs cycle enzyme, citrate synthase, were decreased in mitochondria from DRG of 22-week STZ-diabetic rats compared with control. ROS levels in perikarya of DRG neurons were not altered by diabetes, but ROS generation from mitochondria treated with antimycin A was diminished compared with control. Reduced mitochondrial respiratory function was associated with downregulation of expression of mitochondrial proteins.

Conclusions: Mitochondrial dysfunction in sensory neurons from type 1 diabetic rats is associated with impaired rates of respiratory activity and occurs without a significant rise in perikaryal ROS. • Keywords: Dorsal root ganglia, Diabetes

• O2k-Network Lab: CA Winnipeg Fernyhough P

Labels: MiParea: Respiration Pathology: Diabetes Stress:Oxidative stress;RONS, Mitochondrial disease Organism: Rat Tissue;cell: Nervous system Preparation: Isolated mitochondria

Coupling state: LEAK, OXPHOS Pathway: N, CIV HRR: Oxygraph-2k

@@ Line 1: / Line 1: @@
 {{Publication
-|title=Roy Chowdhury SK, Zherebitskaya E, Smith DR, Akude E, Chattopadhyay S, Jolivalt CG, Calcutt NA, Fernyhough P (2010) Mitochondrial respiratory chain dysfunction in dorsal root ganglia of streptozotocin-induced diabetic rats and its correction by insulin treatment. Diabetes 59:1082-91.
+|title=Chowdhury SK, Zherebitskaya E, Smith DR, Akude E, Chattopadhyay S, Jolivalt CG, Calcutt NA, Fernyhough P (2010) Mitochondrial respiratory chain dysfunction in dorsal root ganglia of streptozotocin-induced diabetic rats and its correction by insulin treatment. Diabetes 59:1082-91.
 |info=[http://www.ncbi.nlm.nih.gov/pubmed/20103706 PMID: 20103706 Open Access]
-|authors=Roy Chowdhury SK, Zherebitskaya E, Smith DR, Akude E, Chattopadhyay S, Jolivalt CG, Calcutt NA, Fernyhough P
+|authors=Chowdhury SK, Zherebitskaya E, Smith DR, Akude E, Chattopadhyay S, Jolivalt CG, Calcutt NA, Fernyhough P
 |year=2010
 |journal=Diabetes
@@ Line 13: / Line 13: @@
 '''Conclusions''': Mitochondrial dysfunction in sensory neurons from type 1 diabetic rats is associated with impaired rates of respiratory activity and occurs without a significant rise in perikaryal ROS.
 |keywords=Dorsal root ganglia, Diabetes
-|mipnetlab=CZ Prague Houstek J, CA Winnipeg Fernyhough P
+|mipnetlab=CA Winnipeg Fernyhough P
 }}
 {{Labeling
@@ Line 23: / Line 23: @@
 |diseases=Diabetes
 |couplingstates=LEAK, OXPHOS
-|substratestates=CI, CIV
+|pathways=N, CIV
 |instruments=Oxygraph-2k
 }}