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| The PVC membranes of the ISE are generally only suitable for operation in aqueous media and are damaged by non-aqueous solvents. Therefore, the necessary washing steps between experiments have to be carefully optimized according to specific experimental regimes [[MiPNet15.03]].
| | #REDIRECT [[MiPNet15.03 O2k-MultiSensor-ISE]] |
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| ===Standard cleaning procedure for TPP+ and reference electrodes===
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| *Rinse the electrodes after use with water, dry with paper, rinse with absolute ethanol, again dry with paper and rinse with water again [[MiPNet15.03]].
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| *Store in a separate falcon tube with a proper storage solution [[MiPNet15.03]]
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| ===After using inhibitors and uncouplers===
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| It is suggested to put the electrodes rinsed with standard cleaning procedure into a falcon tube filled with distiled water for a while (15-30 min) and then put to the falcon tube with liver homogenate for 20-30 min. (Defrosted homogenate, mitochondria of cell cultures from previous experiments could be used for this purpose).
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| Alternatively, the electrodes could be washed with liver homogenate or old mitochondria together with the chamber (inserted into the chamber like in an experiment).
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| '''!!! This procedure is probably absolutely necessary to get rid of all the inhibitors !!!'''
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| If this does not remove the inhibition problem the third option suggested in the manual [[G MiPNet15.03 MultiSensor-ISE]] is to actually immerse the TPP electrode in absolute ethanol for a few minutes (in the chamber, with stirring). However, afterwards the electrodes have to be tested and a reduced lifetime for the membranes is to be expected.
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| Please see also the [[Talk:Cleaning the TPP+ electrodes|Discussion page]]!
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| {{#set:Technical service=Membrane potential|Technical Service=pX signal}}
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| <br> {{Technical service}}
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