Difference between revisions of "Costa 2013 Cell Death Dis"
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|title=Costa AC, Loh SH, Martins LM (2013) ''Drosophila'' Trap1 protects against mitochondrial dysfunction in a PINK1/parkin model of Parkinson's disease. Cell Death Dis 4:e467. | |title=Costa AC, Loh SH, Martins LM (2013) ''Drosophila'' Trap1 protects against mitochondrial dysfunction in a PINK1/parkin model of Parkinson's disease. Cell Death Dis 4:e467. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed?term=Drosophila%20Trap1%20protects%20against%20mitochondrial%20dysfunction%20in%20a%20PINK1%2Fparkin%20model%20of%20Parkinson%E2%80%99s%20disease PMID: 23328674] | |info=[http://www.ncbi.nlm.nih.gov/pubmed?term=Drosophila%20Trap1%20protects%20against%20mitochondrial%20dysfunction%20in%20a%20PINK1%2Fparkin%20model%20of%20Parkinson%E2%80%99s%20disease PMID: 23328674 Open Access] | ||
|authors=Costa AC, Loh SH, Martins LM | |authors=Costa AC, Loh SH, Martins LM | ||
|year=2013 | |year=2013 | ||
|journal=Cell Death Dis | |journal=Cell Death Dis | ||
|abstract=Mitochondrial dysfunction caused by protein aggregation has been shown to have an important role in neurological diseases, such as Parkinson's disease (PD). Mitochondria have evolved at least two levels of defence mechanisms that ensure their integrity and the viability of their host cell. First, molecular quality control, through the upregulation of mitochondrial chaperones and proteases, guarantees the clearance of damaged proteins. Second, organellar quality control ensures the clearance of defective mitochondria through their selective autophagy. Studies in ''Drosophila'' have highlighted mitochondrial dysfunction linked with the loss of the PTEN-induced putative kinase 1 (PINK1) as a mechanism of PD pathogenesis. The mitochondrial chaperone TNF receptor-associated protein 1 (TRAP1) was recently reported to be a cellular substrate for the PINK1 kinase. Here, we characterise ''Drosophila'' Trap1 null mutants and describe the genetic analysis of Trap1 function with Pink1 and parkin. We show that loss of Trap1 results in a decrease in mitochondrial function and increased sensitivity to stress, and that its upregulation in neurons of Pink1 mutant rescues mitochondrial impairment. Additionally, the expression of Trap1 was able to partially rescue mitochondrial impairment in parkin mutant flies; and conversely, expression of parkin rescued mitochondrial impairment in Trap1 mutants. We conclude that Trap1 works downstream of Pink1 and in parallel with parkin in ''Drosophila'', and that enhancing its function may ameliorate mitochondrial dysfunction and rescue neurodegeneration in PD. | |abstract=Mitochondrial dysfunction caused by protein aggregation has been shown to have an important role in neurological diseases, such as Parkinson's disease (PD). Mitochondria have evolved at least two levels of defence mechanisms that ensure their integrity and the viability of their host cell. First, molecular quality control, through the upregulation of mitochondrial chaperones and proteases, guarantees the clearance of damaged proteins. Second, organellar quality control ensures the clearance of defective mitochondria through their selective autophagy. Studies in ''Drosophila'' have highlighted mitochondrial dysfunction linked with the loss of the PTEN-induced putative kinase 1 (PINK1) as a mechanism of PD pathogenesis. The mitochondrial chaperone TNF receptor-associated protein 1 (TRAP1) was recently reported to be a cellular substrate for the PINK1 kinase. Here, we characterise ''Drosophila'' Trap1 null mutants and describe the genetic analysis of Trap1 function with Pink1 and parkin. We show that loss of Trap1 results in a decrease in mitochondrial function and increased sensitivity to stress, and that its upregulation in neurons of Pink1 mutant rescues mitochondrial impairment. Additionally, the expression of Trap1 was able to partially rescue mitochondrial impairment in parkin mutant flies; and conversely, expression of parkin rescued mitochondrial impairment in Trap1 mutants. We conclude that Trap1 works downstream of Pink1 and in parallel with parkin in ''Drosophila'', and that enhancing its function may ameliorate mitochondrial dysfunction and rescue neurodegeneration in PD. | ||
|keywords=Parkinson’s disease, Drosophila, PTEN-induced putative kinase 1 (PINK1), TNF receptor-associated protein 1 (TRAP1), Stress | |keywords=Parkinson’s disease, ''Drosophila'', PTEN-induced putative kinase 1 (PINK1), TNF receptor-associated protein 1 (TRAP1), Stress | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
Revision as of 11:39, 27 April 2015
| Costa AC, Loh SH, Martins LM (2013) Drosophila Trap1 protects against mitochondrial dysfunction in a PINK1/parkin model of Parkinson's disease. Cell Death Dis 4:e467. |
Costa AC, Loh SH, Martins LM (2013) Cell Death Dis
Abstract: Mitochondrial dysfunction caused by protein aggregation has been shown to have an important role in neurological diseases, such as Parkinson's disease (PD). Mitochondria have evolved at least two levels of defence mechanisms that ensure their integrity and the viability of their host cell. First, molecular quality control, through the upregulation of mitochondrial chaperones and proteases, guarantees the clearance of damaged proteins. Second, organellar quality control ensures the clearance of defective mitochondria through their selective autophagy. Studies in Drosophila have highlighted mitochondrial dysfunction linked with the loss of the PTEN-induced putative kinase 1 (PINK1) as a mechanism of PD pathogenesis. The mitochondrial chaperone TNF receptor-associated protein 1 (TRAP1) was recently reported to be a cellular substrate for the PINK1 kinase. Here, we characterise Drosophila Trap1 null mutants and describe the genetic analysis of Trap1 function with Pink1 and parkin. We show that loss of Trap1 results in a decrease in mitochondrial function and increased sensitivity to stress, and that its upregulation in neurons of Pink1 mutant rescues mitochondrial impairment. Additionally, the expression of Trap1 was able to partially rescue mitochondrial impairment in parkin mutant flies; and conversely, expression of parkin rescued mitochondrial impairment in Trap1 mutants. We conclude that Trap1 works downstream of Pink1 and in parallel with parkin in Drosophila, and that enhancing its function may ameliorate mitochondrial dysfunction and rescue neurodegeneration in PD. • Keywords: Parkinson’s disease, Drosophila, PTEN-induced putative kinase 1 (PINK1), TNF receptor-associated protein 1 (TRAP1), Stress
Labels: MiParea: Respiration, Genetic knockout;overexpression, mt-Medicine
Pathology: Parkinson's
Organism: Drosophila
Preparation: Permeabilized tissue Enzyme: Complex I, Complex II;succinate dehydrogenase Regulation: Inhibitor Coupling state: OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k
