Difference between revisions of "Cyanide"
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|description='''Cyanide''' (usually added as KCN) is a competitive inhibitor of [[Complex_IV| cytochcrome ''c'' oxidase (CIV)]]. Inhibition is reversed by pyruvate and high oxygen levels. | |description='''Cyanide''' (usually added as KCN) is a competitive inhibitor of [[Complex_IV| cytochcrome ''c'' oxidase (CIV)]]. Inhibition is reversed by pyruvate and high oxygen levels. | ||
|info=[[MiPNet09.12]] | |info=[[MiPNet09.12]] | ||
}} | }} | ||
{{MitoPedia O2k and high-resolution respirometry|type=Inhibitor ETS | {{MitoPedia O2k and high-resolution respirometry|type=Inhibitor ETS | ||
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{{MitoPedia topics | {{MitoPedia topics | ||
|mitopedia topic=Inhibitor | |mitopedia topic=Inhibitor | ||
}} | }} | ||
__TOC__ | __TOC__ | ||
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=== Inhibitor titrations, CIV-assay, and ROX === | === Inhibitor titrations, CIV-assay, and ROX === | ||
* Cyanide and determination of [[Residual oxygen consumption|residual oxygen consumption (ROX)]]. | ::::* Cyanide and determination of [[Residual oxygen consumption|residual oxygen consumption (ROX)]]. | ||
* [[Complex IV|Complex IV assay]] | ::::* [[Complex IV|Complex IV assay]] | ||
'''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | ::: '''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | ||
::* Titration volume: 2 µl of 1 M stock, using a 10 µl syringe (2 ml O2k-chamber). | ::::* Titration volume: 2 µl of 1 M stock, using a 10 µl syringe (2 ml O2k-chamber). | ||
::* Final concentration: 1 mM. | ::::* Final concentration: 1 mM. | ||
=== Preparation of 1 M KCN stock === | === Preparation of 1 M KCN stock === | ||
'''Kcn'''(KCN): Fluka 60178, 100 g, store at R.T.; FW = 65.12. | :::: '''Kcn'''(KCN): Fluka 60178, 100 g, store at R.T.; FW = 65.12. | ||
<span style="color:#8B008B"> '''Caution:''' KCN is photosensitive and hygroscopic; HCN is volatile and very toxic! Prepare the solution fresh everyday!</span> | :::: <span style="color:#8B008B"> '''Caution:''' KCN is photosensitive and hygroscopic; HCN is volatile and very toxic! Prepare the solution fresh everyday!</span> | ||
:: | ::::# Weigh 13 mg of KCN. | ||
:: | ::::# Dissolve in 0.2 ml H<sub>2</sub>O. | ||
:: | ::::# The pH of the solution may be very alkaline - adjust to physiological pH with HCl. | ||
Revision as of 09:55, 2 June 2016
- high-resolution terminology - matching measurements at high-resolution
Cyanide
Description
Cyanide (usually added as KCN) is a competitive inhibitor of cytochcrome c oxidase (CIV). Inhibition is reversed by pyruvate and high oxygen levels.
Abbreviation: KCN
Reference: MiPNet09.12
MitoPedia topics: Inhibitor
Application in HRR
Inhibitor titrations, CIV-assay, and ROX
- Cyanide and determination of residual oxygen consumption (ROX).
- Complex IV assay
- O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 2 µl of 1 M stock, using a 10 µl syringe (2 ml O2k-chamber).
- Final concentration: 1 mM.
- O2k manual titrations MiPNet09.12 O2k-Titrations
Preparation of 1 M KCN stock
- Kcn(KCN): Fluka 60178, 100 g, store at R.T.; FW = 65.12.
- Caution: KCN is photosensitive and hygroscopic; HCN is volatile and very toxic! Prepare the solution fresh everyday!
- Weigh 13 mg of KCN.
- Dissolve in 0.2 ml H2O.
- The pH of the solution may be very alkaline - adjust to physiological pH with HCl.
