Difference between revisions of "Cyanide"
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::::* [[alternative oxidase]] | ::::* [[alternative oxidase]] | ||
=== Preparation of KCN stock === | === Preparation of KCN stock === | ||
:::: '''Kcn'''(KCN): Fluka 60178, 100 g, store at R.T.; M = 65.12 g·mol<sup>-1</sup>. | :::: '''Kcn'''(KCN): Fluka 60178, 100 g, store at R.T.; M = 65.12 g·mol<sup>-1</sup>. | ||
:::: '''Caution:''' In April 2019 a change in the procedure for preparing the KCN was done that recommended to prepare 20 mM KCN stock and titrate 100 µL. 2021-01-22: after discussions on KCN toxicity we recommend to prepare of 0.5 M KCN stock solutions. | |||
:::: <span style="color:#8B008B"> '''Caution:''' KCN is photosensitive and hygroscopic; HCN is volatile and very toxic! </span> | :::: <span style="color:#8B008B"> '''Caution:''' KCN is photosensitive and hygroscopic; HCN is volatile and very toxic! </span> | ||
Revision as of 11:12, 9 March 2021
- high-resolution terminology - matching measurements at high-resolution
Cyanide
Description
Cyanide (usually added as KCN) is a competitive inhibitor of cytochcrome c oxidase (CIV). Inhibition is reversed by pyruvate and high oxygen levels.
Abbreviation: KCN
Reference: MiPNet09.12
MitoPedia topics: Inhibitor
Application in HRR
Last update 2021-01-22
Inhibitor titrations, CIV-assay, alternative oxidase and ROX
- Cyanide and determination of residual oxygen consumption (ROX).
- Complex IV assay
- alternative oxidase
Preparation of KCN stock
- Kcn(KCN): Fluka 60178, 100 g, store at R.T.; M = 65.12 g·mol-1.
- Caution: In April 2019 a change in the procedure for preparing the KCN was done that recommended to prepare 20 mM KCN stock and titrate 100 µL. 2021-01-22: after discussions on KCN toxicity we recommend to prepare of 0.5 M KCN stock solutions.
- Caution: KCN is photosensitive and hygroscopic; HCN is volatile and very toxic!
- Preparation of 0.5 M KCN stock solution (dissolved in H2O):
- Weigh 32.6 mg of KCN.
- Dissolve in 1 ml H2O.
- The pH of the solution is basic. Since the titration amount is low and HCN may evaporate during pH adjustment with HCl, the pH is not adjusted.
- Divide into 0.2 mL portions
- Store at -20 °C.
- » O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 4 µL using a 10 µL Hamilton syringe.
- Titration volume (0.5-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
- Final concentration: 1 mM.
- » O2k manual titrations MiPNet09.12 O2k-Titrations
MitoPedia topics: Inhibitor
