Difference between revisions of "Cyanide"

Revision as of 10:23, 18 May 2012

high-resolution terminology - matching measurements at high-resolution

Cyanide

Potassium cyanide is a competitive inhibitor of cytochcrome c oxidase (CIV). Inhibition is reversed by pyruvate and high oxygen levels.

Abbreviation: Kcn

Reference: MiPNet09.12

MitoPedia topics: Inhibitor

Kcn: Potassium cyanide (KCN); Fluka 60178, 100 g, store at R.T.; FW = 65.12

Caution: KCN is photosensitive, hygroscopic and very toxic! Prepare the solution fresh everyday!

Preparation of 1 M stock solution (dissolved H₂O):

1) weigh 13 mg of Potassium cyanide

2) dissolve in 0.2 ml H₂O

3) the pH of the solution may be very alkaline - adjust with HCl

Oxygraph-2k Manual Titrations: MiPNet09.12

@@ Line 1: / Line 1: @@
 {{MitoPedia
 |abbr=Kcn
-|description=Competitive inhibitor of [[cytochrome c oxidase | cytochcrome ''c'' oxidase (CIV)]]. Inhibition is reversed by pyruvate and high oxygen levels.
+|description=Potassium cyanide is a competitive inhibitor of [[cytochrome c oxidase | cytochcrome ''c'' oxidase (CIV)]]. Inhibition is reversed by pyruvate and high oxygen levels.
 |info=[[MiPNet09.12]]
 |type=Inhibitor ETS
@@ Line 9: / Line 9: @@
 |type=Inhibitor ETS
 }}
+== Application in [[HRR]] ==
+'''Kcn: Potassium cyanide''' (KCN); Fluka 60178, 100 g, store at R.T.; FW = 65.12
+<span style="color:#8B008B"> '''Caution:''' KCN is photosensitive, hygroscopic and very toxic! Prepare the solution fresh everyday!</span>
+'''Preparation of 1 M stock solution''' (dissolved H<sub>2</sub>O):
+::1) weigh 13 mg of Potassium cyanide
+::2) dissolve in 0.2 ml H<sub>2</sub>O
+::3) the pH of the solution may be very alkaline - adjust with HCl
+'''Oxygraph-2k Manual Titrations:'''  [[MiPNet09.12]]
+::* Titration volume: 2 µl using a 10 µl syringe
+::* Final conc. in 2 ml O2k-chamber: 1 mM