Difference between revisions of "Cyanide"
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Revision as of 17:09, 8 June 2012
- high-resolution terminology - matching measurements at high-resolution
Cyanide
Description
Potassium cyanide is a competitive inhibitor of cytochcrome c oxidase (CIV). Inhibition is reversed by pyruvate and high oxygen levels.
Abbreviation: KCN
Reference: MiPNet09.12
MitoPedia topics:
Inhibitor
Application in HRR
Kcn: Potassium cyanide (KCN); Fluka 60178, 100 g, store at R.T.; FW = 65.12
Caution: KCN is photosensitive, hygroscopic and very toxic! Prepare the solution fresh everyday!
Preparation of 1 M stock solution (dissolved H2O):
- 1) weigh 13 mg of Potassium cyanide
- 2) dissolve in 0.2 ml H2O
- 3) the pH of the solution may be very alkaline - adjust with HCl
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 2 µl using a 10 µl syringe
- Final conc. in 2 ml O2k-chamber: 1 mM
