Difference between revisions of "Cyanide"
From Bioblast
m (moved Potassium cyanide to Cyanide) |
|||
Line 1: | Line 1: | ||
{{MitoPedia | {{MitoPedia | ||
|abbr=KCN | |abbr=KCN | ||
|description=''' | |description='''Cyanide''' is a competitive inhibitor of [[cytochrome c oxidase | cytochcrome ''c'' oxidase (CIV)]]. Inhibition is reversed by pyruvate and high oxygen levels. | ||
|info=[[MiPNet09.12]] | |info=[[MiPNet09.12]] | ||
|type=Inhibitor ETS | |type=Inhibitor ETS | ||
}} | |||
{{MitoPedia methods|type=Inhibitor ETS | |||
}} | }} | ||
{{MitoPedia topics | {{MitoPedia topics | ||
Line 9: | Line 11: | ||
|type=Inhibitor ETS | |type=Inhibitor ETS | ||
}} | }} | ||
== Application in [[HRR]] == | == Application in [[HRR]] == | ||
Revision as of 17:10, 8 June 2012
Description
Cyanide is a competitive inhibitor of cytochcrome c oxidase (CIV). Inhibition is reversed by pyruvate and high oxygen levels.
Abbreviation: KCN
Reference: MiPNet09.12
MitoPedia topics: Inhibitor
Application in HRR
Kcn: Potassium cyanide (KCN); Fluka 60178, 100 g, store at R.T.; FW = 65.12
Caution: KCN is photosensitive, hygroscopic and very toxic! Prepare the solution fresh everyday!
Preparation of 1 M stock solution (dissolved H2O):
- 1) weigh 13 mg of Potassium cyanide
- 2) dissolve in 0.2 ml H2O
- 3) the pH of the solution may be very alkaline - adjust with HCl
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 2 Β΅l using a 10 Β΅l syringe
- Final conc. in 2 ml O2k-chamber: 1 mM