Difference between revisions of "Cyanide"

Revision as of 17:19, 8 June 2012

high-resolution terminology - matching measurements at high-resolution

Cyanide

Cyanide is a competitive inhibitor of cytochcrome c oxidase (CIV). Inhibition is reversed by pyruvate and high oxygen levels.

Abbreviation: KCN

Reference: MiPNet09.12

MitoPedia methods: Respirometry

MitoPedia topics: Inhibitor

Kcn(KCN): Fluka 60178, 100 g, store at R.T.; FW = 65.12

Caution: KCN is photosensitive and hygroscopic; HCN is volatile and very toxic! Prepare the solution fresh everyday!

Preparation of 1 M stock solution (dissolved H₂O):

1) Weigh 13 mg of Potassium cyanide

2) Dissolve in 0.2 ml H₂O

3) The pH of the solution may be very alkaline - adjust to physiological pH with HCl

Oxygraph-2k Manual Titrations: MiPNet09.12

@@ Line 4: / Line 4: @@
 |info=[[MiPNet09.12]]
 |type=Inhibitor ETS
-}}
+}}{{MitoPedia methods
-{{MitoPedia methods|type=Inhibitor ETS
+|mitopedia method=Respirometry
-}}
+|type=Inhibitor ETS
-{{MitoPedia topics
+}}{{MitoPedia topics
 |mitopedia topic=Inhibitor
 |type=Inhibitor ETS
@@ Line 13: / Line 13: @@
 == Application in [[HRR]] ==
-'''Kcn: Potassium cyanide''' (KCN); Fluka 60178, 100 g, store at R.T.; FW = 65.12
+'''Kcn'''(KCN): Fluka 60178, 100 g, store at R.T.; FW = 65.12
-<span style="color:#8B008B"> '''Caution:''' KCN is photosensitive, hygroscopic and very toxic! Prepare the solution fresh everyday!</span>
+<span style="color:#8B008B"> '''Caution:''' KCN is photosensitive and hygroscopic; HCN is volatile and very toxic! Prepare the solution fresh everyday!</span>
 '''Preparation of 1 M stock solution''' (dissolved H<sub>2</sub>O):
-::1) weigh 13 mg of Potassium cyanide
+::1) Weigh 13 mg of Potassium cyanide
-::2) dissolve in 0.2 ml H<sub>2</sub>O
+::2) Dissolve in 0.2 ml H<sub>2</sub>O
-::3) the pH of the solution may be very alkaline - adjust with HCl
+::3) The pH of the solution may be very alkaline - adjust to physiological pH with HCl