Difference between revisions of "Cyanide"
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{{MitoPedia | {{MitoPedia | ||
|abbr=KCN | |abbr=KCN | ||
|description='''Cyanide''' is a competitive inhibitor of [[cytochrome c oxidase | cytochcrome ''c'' oxidase (CIV)]]. Inhibition is reversed by pyruvate and high oxygen levels. | |description='''Cyanide''' (usuall added as KCN) is a competitive inhibitor of [[cytochrome c oxidase | cytochcrome ''c'' oxidase (CIV)]]. Inhibition is reversed by pyruvate and high oxygen levels. | ||
|info=[[MiPNet09.12]] | |info=[[MiPNet09.12]] | ||
|type=Inhibitor ETS | |type=Inhibitor ETS | ||
}}{{MitoPedia methods | }} | ||
{{MitoPedia methods | |||
|mitopedia method=Respirometry | |mitopedia method=Respirometry | ||
|type=Inhibitor ETS | |type=Inhibitor ETS | ||
}}{{MitoPedia topics | }} | ||
{{MitoPedia topics | |||
|mitopedia topic=Inhibitor | |mitopedia topic=Inhibitor | ||
|type=Inhibitor ETS | |type=Inhibitor ETS | ||
Revision as of 17:21, 8 June 2012
- high-resolution terminology - matching measurements at high-resolution
Cyanide
Description
Cyanide (usuall added as KCN) is a competitive inhibitor of cytochcrome c oxidase (CIV). Inhibition is reversed by pyruvate and high oxygen levels.
Abbreviation: KCN
Reference: MiPNet09.12
MitoPedia methods:
Respirometry
MitoPedia topics:
Inhibitor
Application in HRR
Kcn(KCN): Fluka 60178, 100 g, store at R.T.; FW = 65.12
Caution: KCN is photosensitive and hygroscopic; HCN is volatile and very toxic! Prepare the solution fresh everyday!
Preparation of 1 M stock solution (dissolved H2O):
- 1) Weigh 13 mg of Potassium cyanide
- 2) Dissolve in 0.2 ml H2O
- 3) The pH of the solution may be very alkaline - adjust to physiological pH with HCl
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 2 µl using a 10 µl syringe
- Final conc. in 2 ml O2k-chamber: 1 mM
