Difference between revisions of "Cyanide"
From Bioblast
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
=== Inhibitor titrations, CIV-assay, and ROX === | |||
* Cyanide and determination of [[Residual oxygen consumption|residual oxygen consumption (ROX)]]. | |||
* [[Complex IV|Complex IV assay]] | |||
* Oxygraph-2k Manual Titrations: [[MiPNet09.12]] | |||
::* Final conc. in 2 ml O2k-chamber: 1 mM | |||
::* Titration volume: 2 µl of 1 M stock, using a 10 µl syringe. | |||
=== Preparation of 1 M KCN stock === | |||
'''Kcn'''(KCN): Fluka 60178, 100 g, store at R.T.; FW = 65.12 | '''Kcn'''(KCN): Fluka 60178, 100 g, store at R.T.; FW = 65.12 | ||
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<span style="color:#8B008B"> '''Caution:''' KCN is photosensitive and hygroscopic; HCN is volatile and very toxic! Prepare the solution fresh everyday!</span> | <span style="color:#8B008B"> '''Caution:''' KCN is photosensitive and hygroscopic; HCN is volatile and very toxic! Prepare the solution fresh everyday!</span> | ||
::1) Weigh 13 mg of KCN | |||
::1) Weigh 13 mg of | |||
::2) Dissolve in 0.2 ml H<sub>2</sub>O | ::2) Dissolve in 0.2 ml H<sub>2</sub>O | ||
::3) The pH of the solution may be very alkaline - adjust to physiological pH with HCl | ::3) The pH of the solution may be very alkaline - adjust to physiological pH with HCl | ||
Revision as of 17:56, 8 June 2012
- high-resolution terminology - matching measurements at high-resolution
Cyanide
Description
Cyanide (usuall added as KCN) is a competitive inhibitor of cytochcrome c oxidase (CIV). Inhibition is reversed by pyruvate and high oxygen levels.
Abbreviation: KCN
Reference: MiPNet09.12
MitoPedia methods:
Respirometry
MitoPedia topics:
Inhibitor
Application in HRR
Inhibitor titrations, CIV-assay, and ROX
- Cyanide and determination of residual oxygen consumption (ROX).
- Complex IV assay
- Oxygraph-2k Manual Titrations: MiPNet09.12
- Final conc. in 2 ml O2k-chamber: 1 mM
- Titration volume: 2 µl of 1 M stock, using a 10 µl syringe.
Preparation of 1 M KCN stock
Kcn(KCN): Fluka 60178, 100 g, store at R.T.; FW = 65.12
Caution: KCN is photosensitive and hygroscopic; HCN is volatile and very toxic! Prepare the solution fresh everyday!
- 1) Weigh 13 mg of KCN
- 2) Dissolve in 0.2 ml H2O
- 3) The pH of the solution may be very alkaline - adjust to physiological pH with HCl
