Difference between revisions of "Cyanide"
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* [[Complex IV|Complex IV assay]] | * [[Complex IV|Complex IV assay]] | ||
''' | '''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | ||
::* Titration volume: 2 µl of 1 M stock, using a 10 µl syringe (2 ml O2k-chamber). | ::* Titration volume: 2 µl of 1 M stock, using a 10 µl syringe (2 ml O2k-chamber). | ||
::* Final concentration: 1 mM. | ::* Final concentration: 1 mM. | ||
Revision as of 11:23, 13 May 2015
- high-resolution terminology - matching measurements at high-resolution
Cyanide
Description
Cyanide (usually added as KCN) is a competitive inhibitor of cytochcrome c oxidase (CIV). Inhibition is reversed by pyruvate and high oxygen levels.
Abbreviation: KCN
Reference: MiPNet09.12
MitoPedia methods:
Respirometry
MitoPedia topics:
Inhibitor
Application in HRR
Inhibitor titrations, CIV-assay, and ROX
- Cyanide and determination of residual oxygen consumption (ROX).
- Complex IV assay
O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume: 2 µl of 1 M stock, using a 10 µl syringe (2 ml O2k-chamber).
- Final concentration: 1 mM.
Preparation of 1 M KCN stock
Kcn(KCN): Fluka 60178, 100 g, store at R.T.; FW = 65.12.
Caution: KCN is photosensitive and hygroscopic; HCN is volatile and very toxic! Prepare the solution fresh everyday!
- 1) Weigh 13 mg of KCN.
- 2) Dissolve in 0.2 ml H2O.
- 3) The pH of the solution may be very alkaline - adjust to physiological pH with HCl.
