Cyanide

high-resolution terminology - matching measurements at high-resolution

Cyanide

Description

Cyanide (usually added as KCN) is a competitive inhibitor of cytochcrome c oxidase (CIV). Inhibition is reversed by pyruvate and high oxygen levels.

Abbreviation: KCN

Reference: MiPNet09.12

MitoPedia topics: Inhibitor 

Application in HRFR

Inhibitor titrations, CIV-assay, and ROX

• Cyanide and determination of residual oxygen consumption (ROX).
• Complex IV assay

O2k manual titrations MiPNet09.12 O2k-Titrations

• Titration volume: 2 µl of 1 M stock, using a 10 µl syringe (2 ml O2k-chamber).
• Final concentration: 1 mM.

Preparation of 1 M KCN stock

Kcn(KCN): Fluka 60178, 100 g, store at R.T.; FW = 65.12.

Caution: KCN is photosensitive and hygroscopic; HCN is volatile and very toxic! Prepare the solution fresh everyday!

1. Weigh 13 mg of KCN.
2. Dissolve in 0.2 ml H₂O.
3. The pH of the solution may be very alkaline - adjust to physiological pH with HCl.