- high-resolution terminology - matching measurements at high-resolution
Cyanide
Description
Cyanide is a competitive inhibitor of cytochcrome c oxidase (CIV). Inhibition is reversed by pyruvate and high oxygen levels.
Abbreviation: KCN
Reference: MiPNet09.12
MitoPedia methods: Respirometry
MitoPedia topics: Inhibitor
Application in HRR
Kcn(KCN): Fluka 60178, 100 g, store at R.T.; FW = 65.12
Caution: KCN is photosensitive and hygroscopic; HCN is volatile and very toxic! Prepare the solution fresh everyday!
Preparation of 1 M stock solution (dissolved H2O):
- 1) Weigh 13 mg of Potassium cyanide
- 2) Dissolve in 0.2 ml H2O
- 3) The pH of the solution may be very alkaline - adjust to physiological pH with HCl
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 2 µl using a 10 µl syringe
- Final conc. in 2 ml O2k-chamber: 1 mM
