D'Onofrio 2020 Sci Rep: Difference between revisions
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{{Publication | {{Publication | ||
|title=D'Onofrio Nunzia, Cacciola Nunzio Antonio, Martino Elisa, Borrelli Francesca, Fiorino Ferdinando, Lombardi Assunta, Neglia Gianluca, Balestrieri Maria Luisa, Campanile Giuseppe (2020) ROS-mediated apoptotic cell death of human colon cancer LoVo cells by milk ฮด-valerobetaine . Sci Rep 10:8978. | |title=D'Onofrio Nunzia, Cacciola Nunzio Antonio, Martino Elisa, Borrelli Francesca, Fiorino Ferdinando, Lombardi Assunta, Neglia Gianluca, Balestrieri Maria Luisa, Campanile Giuseppe (2020) ROS-mediated apoptotic cell death of human colon cancer LoVo cells by milk ฮด-valerobetaine. Sci Rep 10:8978. | ||
|info=[https://www.ncbi.nlm.nih.gov/pubmed/32488123 PMID: 32488123 Open Access] | |info=[https://www.ncbi.nlm.nih.gov/pubmed/32488123 PMID: 32488123 Open Access] | ||
|authors=D'Onofrio Nunzia, Cacciola Nunzio Antonio, Martino Elisa, Borrelli Francesca, Fiorino Ferdinando, Lombardi Assunta, Neglia Gianluca, Balestrieri Maria Luisa, Campanile Giuseppe | |authors=D'Onofrio Nunzia, Cacciola Nunzio Antonio, Martino Elisa, Borrelli Francesca, Fiorino Ferdinando, Lombardi Assunta, Neglia Gianluca, Balestrieri Maria Luisa, Campanile Giuseppe | ||
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}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration | |area=Respiration, Pharmacology;toxicology | ||
|diseases=Cancer | |||
|organism=Human | |||
|tissues=Endothelial;epithelial;mesothelial cell | |||
|preparations=Intact cells | |||
|topics=Cyt c | |||
|pathways=CIV | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional=2020-06 | |additional=2020-06 | ||
}} | }} | ||
Revision as of 19:31, 8 June 2020
| D'Onofrio Nunzia, Cacciola Nunzio Antonio, Martino Elisa, Borrelli Francesca, Fiorino Ferdinando, Lombardi Assunta, Neglia Gianluca, Balestrieri Maria Luisa, Campanile Giuseppe (2020) ROS-mediated apoptotic cell death of human colon cancer LoVo cells by milk ฮด-valerobetaine. Sci Rep 10:8978. |
D'Onofrio Nunzia, Cacciola Nunzio Antonio, Martino Elisa, Borrelli Francesca, Fiorino Ferdinando, Lombardi Assunta, Neglia Gianluca, Balestrieri Maria Luisa, Campanile Giuseppe (2020) Sci Rep
Abstract: ฮด-Valerobetaine (ฮดVB) is a constitutive milk metabolite with antioxidant and anti-inflammatory activities. Here, we tested the antineoplastic properties of milk ฮดVB on human colorectal cancer cells. CCD 841 CoN (non-tumorigenic), HT-29 (p53 mutant adenocarcinoma) and LoVo (APC/RAS mutant adenocarcinoma) cells were exposed to 3 kDa milk extract, ฮดVB (2 mM) or milk+ฮดVB up to 72 h. Results showed a time- and dose-dependent capability of ฮดVB to inhibit cancer cell viability, with higher potency in LoVo cells. Treatment with milk+ฮดVB arrested cell cycle in G2/M and SubG1 phases by upregulating p21, cyclin A, cyclin B1 and p53 protein expressions. Noteworthy, ฮดVB also increased necrosis (P < 0.01) and when used in combination with milk it improved its activity on live cell reduction (P < 0.05) and necrosis (P < 0.05). ฮดVB-enriched milk activated caspase 3, caspase 9, Bax/Bcl-2 apoptotic pathway and reactive oxygen species (ROS) production, whereas no effects on ROS generation were observed in CCD 841 CoN cells. The altered redox homeostasis induced by milk+ฮดVB was accompanied by upregulation of sirtuin 6 (SIRT6). SIRT6 silencing by small interfering RNA blocked autophagy and apoptosis activated by milk+ฮดVB, unveiling the role of this sirtuin in the ROS-mediated apoptotic LoVo cell death.
โข Bioblast editor: Plangger M
Labels: MiParea: Respiration, Pharmacology;toxicology
Pathology: Cancer
Organism: Human Tissue;cell: Endothelial;epithelial;mesothelial cell Preparation: Intact cells
Regulation: Cyt c
Pathway: CIV HRR: Oxygraph-2k
2020-06
