Difference between revisions of "DatLab and SUIT protocols"
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|description=This is a brief summary of steps to be taken for performing a high-resolution respirometry experiment with '''[[SUIT protocols]]''' using the OROBOROS [[Oxygraph-2k]] and '''[[DatLab]]''' software. (1) Search for a specific [[SUIT protocol name]] (go to [[MitoPedia:_SUIT#SUIT_protocols |MitoPedia: SUIT]]). The list of MitoPedia SUIT protocols can be sorted by the [[SUIT protocol category]] (sorting by SUIT protocol name), which is listed as the 'abbreviation' of the SUIT protocol name. (2) Copy the template for [[Mark names]] into your DatLab subdirectory: DatLab\APPDATA\MTEMPLAT. (3) Copy the [[DatLab-Excel templates |DatLab-Excel template]] for this SUIT protocol. (4) Follow the link to the corresponding publication or MiPNet communication, where the pdf file describing the SUIT protocol is available. After each sequential titration, a mark is set on the plot for flux or flow. After having set all marks, pull down the 'Mark names' menu, select the corresponding SUIT protocol for mark names, and rename all marks. The Mark names template also provides standard values of the titration volume preceding each mark. (5) Go to 'Mark statistics' [F2], copy to clipboard, and paste into the sample tab. | |description=This is a brief summary of steps to be taken for performing a high-resolution respirometry experiment with '''[[SUIT protocols]]''' using the OROBOROS [[Oxygraph-2k]] and '''[[DatLab]]''' software. (1) Search for a specific [[SUIT protocol name]] (go to [[MitoPedia:_SUIT#SUIT_protocols |MitoPedia: SUIT]]). The list of MitoPedia SUIT protocols can be sorted by the [[SUIT protocol category]] (sorting by SUIT protocol name), which is listed as the 'abbreviation' of the SUIT protocol name. (2) Copy the template for [[Mark names]] into your DatLab subdirectory: DatLab\APPDATA\MTEMPLAT. (3) Copy the [[DatLab-Excel templates |DatLab-Excel template]] for this SUIT protocol. (4) Follow the link to the corresponding publication or MiPNet communication, where the pdf file describing the SUIT protocol is available. After each sequential titration, a mark is set on the plot for flux or flow. After having set all marks, pull down the 'Mark names' menu, select the corresponding SUIT protocol for mark names, and rename all marks. The Mark names template also provides standard values of the titration volume preceding each mark. (5) Go to 'Mark statistics' [F2], copy to clipboard, and paste into the sample tab. | ||
: Example: | |||
:* SUIT protocol name: [[SUIT MiPNet12.23 FibreRespiration]] | |||
:* Mark names in DatLab: [[MiPNet12.23 FibreRespiration]] | |||
:* DatLab-Excel template: SUIT_MiPNet12.23_FibreRespiration.xlsx | |||
:* MiPNet communciation: [[MiPNet12.23 FibreRespiration] | |||
|info=[[MitoPedia: DatLab]] | |info=[[MitoPedia: DatLab]] | ||
}} | }} | ||
Revision as of 17:03, 19 July 2016
{{MitoPedia |abbr= |description=This is a brief summary of steps to be taken for performing a high-resolution respirometry experiment with SUIT protocols using the OROBOROS Oxygraph-2k and DatLab software. (1) Search for a specific SUIT protocol name (go to MitoPedia: SUIT). The list of MitoPedia SUIT protocols can be sorted by the SUIT protocol category (sorting by SUIT protocol name), which is listed as the 'abbreviation' of the SUIT protocol name. (2) Copy the template for Mark names into your DatLab subdirectory: DatLab\APPDATA\MTEMPLAT. (3) Copy the DatLab-Excel template for this SUIT protocol. (4) Follow the link to the corresponding publication or MiPNet communication, where the pdf file describing the SUIT protocol is available. After each sequential titration, a mark is set on the plot for flux or flow. After having set all marks, pull down the 'Mark names' menu, select the corresponding SUIT protocol for mark names, and rename all marks. The Mark names template also provides standard values of the titration volume preceding each mark. (5) Go to 'Mark statistics' [F2], copy to clipboard, and paste into the sample tab.
- Example:
- SUIT protocol name: SUIT MiPNet12.23 FibreRespiration
- Mark names in DatLab: MiPNet12.23 FibreRespiration
- DatLab-Excel template: SUIT_MiPNet12.23_FibreRespiration.xlsx
- MiPNet communciation: [[MiPNet12.23 FibreRespiration]
|info=MitoPedia: DatLab }}
MitoPedia concepts: MiP concept, SUIT concept
Contributed by Gnaiger Erich 2016-07-19.
