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Dual wavelength analysis
Description
If a sample contains a number of absorbing substances, it is sometimes possible to select discrete pairs of wavelengths at which the change in absorbance of a particular substance (due to oxidation or reduction, for example) is largely independent of changes in the absorbance of other substances present. Dual wavelength analysis can be carried out for cytochrome c by subtracting the absorbance at 540nm from that at 550nm in order to give a measure of the degree of reduction. Similarly, by subtracting the absorbance at 465nm from that at 444nm, an indicator of the redox state of cytochrome aa3 can be obtained.
Abbreviation: n.a.
MitoPedia methods:
Spectrophotometry
