Dudzinska 2012 Abstract Bioblast
| Dudzinska D, Luzak B, Stanczyk L, Rywaniak J, Watala C (2012) The influence of the extract from Rubus parvifolius leaves (EL17) on expression and activity of apyrase (CD 39) on Human Umbilical Vein Endothelial Cells (HUVECs). Mitochondr Physiol Network 17.12. |
Link: MiPNet17.12 Bioblast 2012 - Open Access
Dudzinska D, Luzak B, Stanczyk L, Rywaniak J, Watala C (2012)
Event: Bioblast 2012
Human Endothelial Cells possess a lot of mechanisms which enable them prevention against thrombosis and plaque formation. One of such preventive systems is antigen CD39 which has the ability to decompose ATP and ADP. This process results in decreased aggregation of blood platelets activated via purinergic pathway. Polyphenols, plant secondary metabolites, are widely known for their antioxidative and cardioprotective activities. Blackberry fruits, which are the rich source of polyphenolic compounds, were proved in the in vivo studies to possess antithrombotic properties. We examined the influence of polyphenolic extract derived from Rubus parvifolius leaves (EL17), on the expression and ADPase activity of CD39 in HUVECs and on blood platelet aggregation. Platelet aggregation was determined in the whole blood obtained from healthy individuals. Whole blood samples were incubated with EL17 at the concentration of 7.5 and 15 ยตg/ml and agonized with 6.4 ยตg/ml ADP. To assess the effect on CD39 expression, HUVECs were incubated for 24 h with EL17 (at the concentration of 1, 5, 10 or 15 ยตg gallic acid per ml), then labeled witch anti-CD39 antibodies, washed (two times) and measured by flow cytometry. To estimate CD39 ADPase activity HUVECs were incubated with EL17 for 24 h and the samples were assayed using Malachite Green method. Platelet aggregation, upon the incubation with 15 ยตg/ml EL17, was inhibited by 19% (132 ยฑ 53 AU vs. 165 ยฑ 52 AU in control without EL17). Moreover, the expression of CD 39 increased significantly after incubation with EL17 (up to 30% and 50% after the treatment with 10 and 15 ยตg/ml EL17, respectively). The ADPase activity in the presence of EL17 remained at the same level as in control. To conclude, the extract from Rubus parvifolius leaves, exerts beneficial influence on platelet reactivity via purinergic pathway and may be considered as a potential antiplatelet modulator in antiplatelet therapy and prevention of cardiovascular diseases.
โข Keywords: Polyphenols, HUVEC, Platelet aggregation
โข O2k-Network Lab: PL Lodz Watala C
Labels:
Tissue;cell: Blood Cell; Suspension Culture"Blood Cell; Suspension Culture" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.
Affiliations and author contributions
Dominika Dudziลska, Bogusลawa Luzak, Lidia Staลczyk, Joanna Rywaniak, Cezary Wataลa
Department of Haemostasis and Haemostatic Disorders, Medical University of Lodz, Lodz, Poland; Email: [email protected]
The poster is supported by the project โProduction of polyphenol extracts of plant origin with antiplatelet and cardioprotective properties โ FLAWOPIRYNAโ, co-financed by European Union from European Regional Development Fund within the frames Innovative Economy Operational Programme.
Figure 1
Effect of EL17 on CD39 expression on HUVECโs surface. Data shown as median and interquartile range (n=14). At higher concentration EL17 increased CD39 expression
