Duicu 2013 Can J Physiol Pharmacol: Difference between revisions
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Revision as of 16:49, 6 August 2013
Duicu OM, Mirica SN, Gheorgheosu DE, Privistirescu AI, Fira-Mladinescu O, Muntean DM (2013) Ageing-induced decrease in cardiac mitochondrial function in healthy rats. Can J Physiol Pharmacol 10.1139/cjpp-2012-0422. |
Duicu OM, Mirica SN, Gheorgheosu DE, Privistirescu AI, Fira-Mladinescu O, Muntean DM (2013) Can J Physiol Pharmacol
Abstract: Background: It is widely recognized that mitochondrial dysfunction is a key component of the multifactorial process of ageing. The effects of age on individual components of mitochondrial function vary across species and strains. In the present study we investigated and compared oxygen consumption, membrane potential (ฮฯ), the sensitivity of the mitochondrial permeability transition pore (mPTP) to calcium overload and production of reactive oxygen species (ROS) in heart mitochondria isolated from old vs. adult healthy Sprague-Dawley (SD) rats. Methods. Respirometry studies and ฮฯ measurements were performed with the Oxygraph-2k (Oroboros, Austria) equipped with a tetraphenylphosphonium electrode. ROS production and calcium retention capacity (CRC) were measured spectrofluorimetrically.
Results: Our results showed an important decline for all bioenergetic parameters for both Complex I and Complex II supported-respiration, a decreased ฮฯ in mitochondria energized with Complex I substrates and an increased mitochondrial ROS production in old vs. the adult group. Mitochondrial sensitivity to Ca2+-induced mtPTP opening was also increased in the old vs. adult animals. Moreover, the protective effect of cyclosporine A on mtPTP opening was significantly reduced in the old group.
Conclusion: Healthy ageing is associated with heart mitochondria dysfunction in Sprague Dawley rats. โข Keywords: Cyclosporine A, heart
โข O2k-Network Lab: RO Timisoara Muntean D
Labels:
Stress:RONS; Oxidative Stress"RONS; Oxidative Stress" is not in the list (Cell death, Cryopreservation, Ischemia-reperfusion, Permeability transition, Oxidative stress;RONS, Temperature, Hypoxia, Mitochondrial disease) of allowed values for the "Stress" property., Aging; Senescence"Aging; Senescence" is not in the list (Cell death, Cryopreservation, Ischemia-reperfusion, Permeability transition, Oxidative stress;RONS, Temperature, Hypoxia, Mitochondrial disease) of allowed values for the "Stress" property. Organism: Rat Tissue;cell: Heart Preparation: Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property. Enzyme: Complex I, Complex II; Succinate Dehydrogenase"Complex II; Succinate Dehydrogenase" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property. Regulation: mt-Membrane potential
HRR: Oxygraph-2k, TPP