Ebanks 2023 PLoS One

Ebanks B, Kwiecinska P, Moisoi N, Chakrabarti L (2023) A method to assess the mitochondrial respiratory capacity of complexes I and II from frozen tissue using the Oroboros O2k-FluoRespirometer. https://doi.org/10.1371/journal.pone.0276147

» PLoS One 18:e0276147. PMID: 37486925 Open Access

Ebanks Brad, Kwiecinska Pola, Moisoi Nicoleta, Chakrabarti Lisa (2023) PLoS One

Abstract: High-resolution respirometry methods allow for the assessment of oxygen consumption by the electron transfer systems within cells, tissue samples, and isolated mitochondrial preparations. As mitochondrial integrity is compromised by the process of cryopreservation, these methods have been limited to fresh samples. Here we present a simple method to assess the activity of mitochondria respiratory complexes I and II in previously cryopreserved murine skeletal muscle tissue homogenates, as well as previously frozen D. melanogaster, as a function of oxygen consumption.

• Bioblast editor: Plangger M • O2k-Network Lab: UK Nottingham Chakrabarti L, UK Leicester Moisoi N

Labels: MiParea: Respiration, Instruments;methods 

Stress:Cryopreservation  Organism: Mouse, Drosophila  Tissue;cell: Skeletal muscle  Preparation: Homogenate 

Coupling state: LEAK, ROUTINE  Pathway: N, S, NS, ROX  HRR: Oxygraph-2k 

2023-07