Endlicher 2009 Acta Medica (Hradec Kralove): Difference between revisions
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|title=Endlicher R, Kriváková P, Lotkova H, Milerová M, Drahota Z, Cervinková Z (2009) Tissue specific sensitivity of mitochondrial permeability transition pore to Ca2+ ions. Acta Medica (Hradec Kralove) 2009;52(2):69-72. | |title=Endlicher R, Kriváková P, Lotkova H, Milerová M, Drahota Z, Cervinková Z (2009) Tissue specific sensitivity of mitochondrial permeability transition pore to Ca2+ ions. Acta Medica (Hradec Kralove) 2009;52(2):69-72. | ||
|info=https://www.ncbi.nlm.nih.gov/pubmed/19777870 | |info=https://www.ncbi.nlm.nih.gov/pubmed/19777870 | ||
|authors=Endlicher R, Krivakova P, Lotkova H, Milerova M, Drahota Z, Cervinkova Z | |authors=Endlicher R, Krivakova P, Lotkova H, Milerova M, Drahota Z, Cervinkova Z | ||
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|abstract=Ca(2+)-induced opening of the mitochondrial permeability transition pore (MPTP) is involved in induction of apoptotic and necrotic processes. We studied sensitivity of MPTP to calcium using the model of Ca(2+)-induced, cyclosporine A-sensitive mitochondrial swelling. Presented data indicate that the extent of mitochondrial swelling (dA520/4 min) induced by addition of 25 microM Ca2+ is seven-fold higher in liver than in heart mitochondria (0.564 +/- 0.08/0.077 +/- 0.01). The extent of swelling induced by 100 microM Ca2+ was in liver tree times higher than in heart mitochondria (0.508 +/- 0.05/ 0.173 +/- 0.02). Cyclosporine A sensitivity showed that opening of the MPTP is involved. We may thus conclude that especially at low Ca2+ concentration heart mitochondria are more resistant to damaging effect of Ca2+ than liver mitochondria. These finding thus support hypothesis that there exist tissue specific strategies of cell protection against induction of the apoptotic and necrotic processes. | |abstract=Ca(2+)-induced opening of the mitochondrial permeability transition pore (MPTP) is involved in induction of apoptotic and necrotic processes. We studied sensitivity of MPTP to calcium using the model of Ca(2+)-induced, cyclosporine A-sensitive mitochondrial swelling. Presented data indicate that the extent of mitochondrial swelling (dA520/4 min) induced by addition of 25 microM Ca2+ is seven-fold higher in liver than in heart mitochondria (0.564 +/- 0.08/0.077 +/- 0.01). The extent of swelling induced by 100 microM Ca2+ was in liver tree times higher than in heart mitochondria (0.508 +/- 0.05/ 0.173 +/- 0.02). Cyclosporine A sensitivity showed that opening of the MPTP is involved. We may thus conclude that especially at low Ca2+ concentration heart mitochondria are more resistant to damaging effect of Ca2+ than liver mitochondria. These finding thus support hypothesis that there exist tissue specific strategies of cell protection against induction of the apoptotic and necrotic processes. | ||
|keywords=Mitochondria; MPTP; Swelling; Cell death; Apoptosis; Necrosis | |keywords=Mitochondria; MPTP; Swelling; Cell death; Apoptosis; Necrosis | ||
|mipnetlab=CZ Hradec Kralove Cervinkova Z, | |mipnetlab=CZ Hradec Kralove Cervinkova Z, | ||
}} | |||
{{Labeling | |||
|diseases=Other | |||
|injuries=Cell death, Permeability transition | |||
|organism=Rat | |||
|tissues=Heart, Liver | |||
|preparations=Isolated mitochondria | |||
}} | }} | ||
Revision as of 10:22, 5 December 2016
| Endlicher R, Kriváková P, Lotkova H, Milerová M, Drahota Z, Cervinková Z (2009) Tissue specific sensitivity of mitochondrial permeability transition pore to Ca2+ ions. Acta Medica (Hradec Kralove) 2009;52(2):69-72. |
» https://www.ncbi.nlm.nih.gov/pubmed/19777870
Endlicher R, Krivakova P, Lotkova H, Milerova M, Drahota Z, Cervinkova Z (2009) Acta Medica (Hradec Kralove)
Abstract: Ca(2+)-induced opening of the mitochondrial permeability transition pore (MPTP) is involved in induction of apoptotic and necrotic processes. We studied sensitivity of MPTP to calcium using the model of Ca(2+)-induced, cyclosporine A-sensitive mitochondrial swelling. Presented data indicate that the extent of mitochondrial swelling (dA520/4 min) induced by addition of 25 microM Ca2+ is seven-fold higher in liver than in heart mitochondria (0.564 +/- 0.08/0.077 +/- 0.01). The extent of swelling induced by 100 microM Ca2+ was in liver tree times higher than in heart mitochondria (0.508 +/- 0.05/ 0.173 +/- 0.02). Cyclosporine A sensitivity showed that opening of the MPTP is involved. We may thus conclude that especially at low Ca2+ concentration heart mitochondria are more resistant to damaging effect of Ca2+ than liver mitochondria. These finding thus support hypothesis that there exist tissue specific strategies of cell protection against induction of the apoptotic and necrotic processes. • Keywords: Mitochondria; MPTP; Swelling; Cell death; Apoptosis; Necrosis
• O2k-Network Lab: CZ Hradec Kralove Cervinkova Z
Labels:
Pathology: Other
Stress:Cell death, Permeability transition
Organism: Rat
Tissue;cell: Heart, Liver
Preparation: Isolated mitochondria
