FCCP: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr=F<sub>''c''</sub> | |abbr=F<sub>''c''</sub> | ||
|description=Carbonyl cyanide p-(trifluoro-methoxy) phenyl-hydrazone (C<sub>10</sub>H<sub>5</sub>F<sub>3</sub>N<sub>4</sub>O); [[uncoupler]], F<sub>''c''</sub> or F''c'': '''FCCP''' added at concentration ''c''. | |description=Carbonyl cyanide p-(trifluoro-methoxy) phenyl-hydrazone (C<sub>10</sub>H<sub>5</sub>F<sub>3</sub>N<sub>4</sub>O); [[uncoupler]], F<sub>''c''</sub> or F''c'': '''FCCP''' added at concentration ''c''; ''c'' is the [[optimum uncoupler concentration]] in titrations of FCCP to obtain maximum mitochondrial respiration in the [[non-coupled respiration|noncoupled]] state of [[ETS capacity]]. | ||
|info=[[MiPNet10.04]]; [[SteinlechnerMaran_1996_AJP]] | |info=[[MiPNet10.04]]; [[SteinlechnerMaran_1996_AJP]] | ||
|type=Uncoupler | |type=Uncoupler |
Revision as of 09:39, 16 November 2011
Description
Carbonyl cyanide p-(trifluoro-methoxy) phenyl-hydrazone (C10H5F3N4O); uncoupler, Fc or Fc: FCCP added at concentration c; c is the optimum uncoupler concentration in titrations of FCCP to obtain maximum mitochondrial respiration in the noncoupled state of ETS capacity.
Abbreviation: Fc
Reference: MiPNet10.04; SteinlechnerMaran_1996_AJP
MitoPedia topics: Uncoupler
Application in HRR
F: FCCP (Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone, C10H5F3N4O); Sigma C 2920, 10 mg, store at 4 ยฐC: FW = 254.17)
Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
Preparation of 1 mM stock solution (dissolved in 99.9% Ethanol):
- 1) weigh 1.27 mg of FCCP into a glass vial and dissolve in 5 ml Ethanol
- 2) divide into 0.2 ml portions OR store in 5 ml glass vial and use subsamples during experiments to avoid contamination of the stock solution
- 3) store in glass vials (recommendation) at -20 ยฐC
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 1 ยตl steps using a 10 ยตl syringe
- Final conc. in 2 ml O2k-chamber: 0.5 ยตM steps
Frequently, more diluted FCCP for getting several titration steps is necessary. In this case prepare more diluted solution (ditution 2-10x) from your stock solution.